Component
Human PLPBP knockout genotype
Engineered loss of PLPBP in human cell lines.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
In the 2023 study, PLP was lower in PLPBP-deficient fibroblasts and HEK293 cells after 96 hours with 20 micromolar PN, PM or PL.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- evidence_location
- Figure 2A-B; Discussion
- experimental_model
- Human patient skin fibroblasts and CRISPR PLPBP-null HEK293 cells.
- exposure
- 20 micromolar individual vitamer for 96 h.
- limitations
- Different conditions may explain the earlier accumulation result.
- nutrient_topic
- Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect. · Vitamin B6
- organism
- Homo sapiens
- plain_language
- The pool response depended on the cell model and culture conditions.
- primary_references
- [ciapaite2023] Maintenance of cellular vitamin B6 levels and mitochondrial oxidative function depend on pyridoxal 5'-phosphate homeostasis protein. (2023). https://pubmed.ncbi.nlm.nih.gov/37451483/ DOI: 10.1016/j.jbc.2023.105047
- tissue_or_cell_type
- Skin fibroblasts and HEK293 cells
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 426–437
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human patient skin fibroblasts and CRISPR PLPBP-null HEK293 cells. · source_derived_draft · unverified_draft
### b6-transport-plpbp-low-plp In the 2023 study, PLP was lower in PLPBP-deficient fibroblasts and HEK293 cells after 96 hours with 20 micromolar PN, PM or PL. Condition category: machinery_impairment nutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect. plain_language: The pool response depended on the cell model and culture conditions. organism: Homo sapiens tissue_or_cell_type: Skin fibroblasts and HEK293 cells experimental_model: Human patient skin fibroblasts and CRISPR PLPBP-null HEK293 cells. limitations: Different conditions may explain the earlier accumulation result. exposure: 20 micromolar individual vitamer for 96 h. evidence_location: Figure 2A-B; Discussion [ciapaite2023] Maintenance of cellular vitamin B6 levels and mitochondrial oxidative function depend on pyridoxal 5'-phosphate homeostasis protein. (2023). https://pubmed.ncbi.nlm.nih.gov/37451483/ DOI: 10.1016/j.jbc.2023.105047
Complete structured claim and evidence
Where it participates (unsigned role)
Forty-eight-hour PDXP siRNA restored PLP toward control concentrations in PLPBP-deficient HEK293 cells and decreased PL.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- evidence_location
- Figure 6B-C
- experimental_model
- Human patient skin fibroblasts and CRISPR PLPBP-null HEK293 cells.
- exposure
- 15 nM siRNA; 48 h; standard PN-containing medium.
- limitations
- Cell experiment; does not prove a direct PLPBP-PDXP interaction.
- nutrient_topic
- Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect. · Vitamin B6
- organism
- Homo sapiens
- plain_language
- Reducing breakdown restored the cellular PLP pool.
- primary_references
- [ciapaite2023] Maintenance of cellular vitamin B6 levels and mitochondrial oxidative function depend on pyridoxal 5'-phosphate homeostasis protein. (2023). https://pubmed.ncbi.nlm.nih.gov/37451483/ DOI: 10.1016/j.jbc.2023.105047
- tissue_or_cell_type
- HEK293 cells
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 439–450
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human patient skin fibroblasts and CRISPR PLPBP-null HEK293 cells. · source_derived_draft · unverified_draft
### b6-transport-pdxp-silencing-plpbp-null Forty-eight-hour PDXP siRNA restored PLP toward control concentrations in PLPBP-deficient HEK293 cells and decreased PL. Condition category: machinery_impairment nutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect. plain_language: Reducing breakdown restored the cellular PLP pool. organism: Homo sapiens tissue_or_cell_type: HEK293 cells experimental_model: Human patient skin fibroblasts and CRISPR PLPBP-null HEK293 cells. limitations: Cell experiment; does not prove a direct PLPBP-PDXP interaction. exposure: 15 nM siRNA; 48 h; standard PN-containing medium. evidence_location: Figure 6B-C [ciapaite2023] Maintenance of cellular vitamin B6 levels and mitochondrial oxidative function depend on pyridoxal 5'-phosphate homeostasis protein. (2023). https://pubmed.ncbi.nlm.nih.gov/37451483/ DOI: 10.1016/j.jbc.2023.105047
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.