Component
Human MCEE-dependent coupled propionyl-CoA pathway activity
Human MCEE-dependent coupled propionyl-CoA pathway activity
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Human MCEE I53R lacked measured activity in the coupled patient-fibroblast assay with overexpressed MMUT and added adenosylcobalamin.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- cross_nutrient
- false
- evidence_location
- Full text Methods 2.6 and Results 3.6; Figure 5
- experimental_model
- MCEE-null human fibroblasts expressing MCEE I53R
- exposure
- I53R expression; MMUT coexpression; 50 micromolar AdoCbl
- limitations
- Overexpression assay; not a clinical test of B12 treatment.
- nutrient_topic
- Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect. · Vitamin B12 (cobalamins)
- organism
- Homo sapiens
- plain_language
- Adding activated B12 did not make this defective epimerase work in the human-cell assay.
- primary_references
- [heuberger-2019-mcee] Genetic, structural, and functional analysis of pathogenic variations causing methylmalonyl-CoA epimerase deficiency. (2019). https://pubmed.ncbi.nlm.nih.gov/30682498/ DOI: 10.1016/j.bbadis.2019.01.021
- tissue_or_cell_type
- Fibroblasts
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Vitamin B12: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 1188–1200
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · MCEE-null human fibroblasts expressing MCEE I53R · source_derived_draft · unverified_draft
### mcee-i53r-reduces-coupled-activity Human MCEE I53R lacked measured activity in the coupled patient-fibroblast assay with overexpressed MMUT and added adenosylcobalamin. Condition category: machinery_impairment nutrient_topic: Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect. plain_language: Adding activated B12 did not make this defective epimerase work in the human-cell assay. organism: Homo sapiens tissue_or_cell_type: Fibroblasts experimental_model: MCEE-null human fibroblasts expressing MCEE I53R limitations: Overexpression assay; not a clinical test of B12 treatment. exposure: I53R expression; MMUT coexpression; 50 micromolar AdoCbl cross_nutrient: false evidence_location: Full text Methods 2.6 and Results 3.6; Figure 5 [heuberger-2019-mcee] Genetic, structural, and functional analysis of pathogenic variations causing methylmalonyl-CoA epimerase deficiency. (2019). https://pubmed.ncbi.nlm.nih.gov/30682498/ DOI: 10.1016/j.bbadis.2019.01.021
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.