Component
Human kidney microsomal CYP3A activity, twenty-donor panel
The renal microsomal preparation in which AM9 formation tracked CYP3A5 protein and the CYP3A5*1 allele. A preparation, not a purified enzyme.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
Microsomes from human kidney produced only AM9, and the rate of formation at 2 and 20 micromolar cyclosporine was positively associated with detectable CYP3A5 protein and with the CYP3A5*1 allele in 4 of the 20 kidneys tested.
Experimental context and source evidence
- duration
- Not stated here
- evidence_access
- Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.
- experimental_model
- Human kidney microsomes from twenty donors
- exposure
- Cyclosporin A at 2 and 20 micromolar
- limitations
- Four of twenty kidneys carried the allele, so the genotype comparison rests on a small group. Ketoconazole at 200 nanomolar inhibited renal AM9 formation by 22 to 55 per cent across 2 to 45 micromolar cyclosporine, which supports a CYP3A route without isolating CYP3A5 from CYP3A4 pharmacologically.
- organism
- Human kidney microsomes from twenty donors
- plain_language
- Microsomes from human kidney produced only AM9, and the rate of formation at 2 and 20 micromolar cyclosporine was positively associated with detectable CYP3A5 protein and with the CYP3A5*1 allele in 4 of the 20 kidneys tested.
- primary_references
- In vitro metabolism of cyclosporine A by human kidney CYP3A5. (2004). https://pubmed.ncbi.nlm.nih.gov/15450954/ DOI: 10.1016/j.bcp.2004.07.012
- route
- In vitro
- tissue
- Renal drug metabolism
Cyclosporine: the complex that inhibits calcineurin, a second cyclophilin, and the transport step that decides exposure (2026-09-23) · lines 212–212
Original AI-assisted curation of seven primary studies resolved by PubMed title search, with every abstract read and all DOIs cross-checked against live PubMed metadata on 2026-09-23. No reference carries a recorded retraction, erratum or expression of concern. Each of the seven is a separate laboratory and each carries its own lineage key, so none of them can be counted twice as independent support. Study-specific concentrations, kinetic constants and limitations retained. Not publisher full text. · supports · Human kidney microsomes from twenty donors · source_derived_draft · unverified_draft
Microsomes from human kidney produced only AM9, and the rate of formation at 2 and 20 micromolar cyclosporine was positively associated with detectable CYP3A5 protein and with the CYP3A5*1 allele in 4 of the 20 kidneys tested.
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.