Component
Basal and maximal respiration in ergothioneine-treated human HeLa cells
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
MPST-deleted HeLa cells showed no significant respiratory increase with ergothioneine versus vehicle.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- evidence_access
- Publisher abstract/introduction plus the authors' article and supplementary legends reproduced in an indexed document. This is source-derived extraction, not raw-data verification. Main-text OCR corrupts some micro-unit symbols; ambiguous doses and binding constants are deliberately not transcribed.
- experimental_condition
- vehicle in MPST-deleted HeLa cells 500 micromolar, 72 hours · L-Ergothioneine Condition belongs to the full experimental contrast; do not separate a joint intervention.
- experimental_condition
- vehicle in MPST-deleted HeLa cells CRISPR-Cas9 deletion · Human mercaptopyruvate sulfurtransferase / MPST Condition belongs to the full experimental contrast; do not separate a joint intervention.
- experimental_contrast
- {"intervention": "ergothioneine in MPST-deleted HeLa cells", "comparator": "vehicle in MPST-deleted HeLa cells", "endpoint": "basal and maximal oxygen consumption", "effect_direction": "no_detected_change", "combination": "joint", "conditions": [{"entity_slug": "ergothioneine", "state": "500 micromolar, 72 hours"}, {"entity_slug": "mpst", "state": "CRISPR-Cas9 deletion"}]} Explicit extracted experimental comparison; source-derived draft.
- experimental_model
- CRISPR-Cas9 MPST-deleted human HeLa cells; 500 micromolar ergothioneine for 72 hours.
- interpretation_status
- Source-derived research curation; not independent raw-data verification.
- limitations
- The comparison is ergothioneine versus vehicle within knockout cells. It does not show that every protective action requires MPST, or that human dietary low intake mimics knockout.
- nutrient_topic
- Ergothioneine mitochondrial supplement; shared molecular requirements are not demonstrated dietary interactions. · L-Ergothioneine
- plain_language
- Loss of the target removed this measured response.
- primary_references
- Sprenger et al. Ergothioneine controls mitochondrial function and exercise performance via direct activation of MPST. DOI 10.1016/j.cmet.2025.01.024; PMID 39965563; https://pubmed.ncbi.nlm.nih.gov/39965563/
- source_locator
- Figures S2E-S2F; Results
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Ergothioneine: mitochondrial transport, MPST and sulfur-handling dependencies (2026-10-02) · lines 81–81
Original AI-assisted curation of five primary studies with publication identifiers, experimental locators and access limitations. Additive chapter supplement, not publisher full text. · supports · CRISPR-Cas9 MPST-deleted human HeLa cells; 500 micromolar ergothioneine for 72 hours. · source_derived_draft · unverified_draft
MPST-deleted HeLa cells showed no significant respiratory increase with ergothioneine versus vehicle.
Complete structured claim and evidenceErgothioneine increased basal and maximal respiration in wild-type human HeLa cells.
Experimental context and source evidence
- dose
- 500 micromolar
- duration
- 72 hours
- evidence_access
- Publisher abstract/introduction plus the authors' article and supplementary legends reproduced in an indexed document. This is source-derived extraction, not raw-data verification. Main-text OCR corrupts some micro-unit symbols; ambiguous doses and binding constants are deliberately not transcribed.
- experimental_condition
- vehicle in wild-type HeLa cells added · L-Ergothioneine Condition belongs to the full experimental contrast; do not separate a joint intervention.
- experimental_contrast
- {"intervention": "500 micromolar ergothioneine for 72 hours", "comparator": "vehicle in wild-type HeLa cells", "endpoint": "basal and maximal oxygen consumption", "effect_direction": "increase", "combination": "single", "conditions": [{"entity_slug": "ergothioneine", "state": "added"}]} Explicit extracted experimental comparison; source-derived draft.
- experimental_model
- Human HeLa cells; 500 micromolar ergothioneine for 72 hours, as stated unambiguously in supplementary Figure S2.
- interpretation_status
- Source-derived research curation; not independent raw-data verification.
- limitations
- Cancer-cell culture at this exposure is not a clinical supplementation result or proof of a nutritional requirement.
- nutrient_topic
- Ergothioneine mitochondrial supplement; shared molecular requirements are not demonstrated dietary interactions. · L-Ergothioneine
- organism
- Homo sapiens
- plain_language
- A human cell model showed a respiratory response.
- primary_references
- Sprenger et al. Ergothioneine controls mitochondrial function and exercise performance via direct activation of MPST. DOI 10.1016/j.cmet.2025.01.024; PMID 39965563; https://pubmed.ncbi.nlm.nih.gov/39965563/
- source_locator
- Figure S2E, wild-type panels
Ergothioneine: mitochondrial transport, MPST and sulfur-handling dependencies (2026-10-02) · lines 73–73
Original AI-assisted curation of five primary studies with publication identifiers, experimental locators and access limitations. Additive chapter supplement, not publisher full text. · supports · Human HeLa cells; 500 micromolar ergothioneine for 72 hours, as stated unambiguously in supplementary Figure S2. · source_derived_draft · unverified_draft
Ergothioneine increased basal and maximal respiration in wild-type human HeLa cells.
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.