Component
Human DIO2 Sec133C/Sec266C experimental variant
Human DIO2 Sec133C/Sec266C experimental variant
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
Where it participates (unsigned role)
Recombinant mouse Wsb1 supported ubiquitination of human DIO2 constructs in a reconstituted assay; disruption of the Wsb1 BC box prevented this effect.
Experimental context and source evidence
- evidence_locator
- Figure 1E-F; Methods: Constructs and ubiquitination assay
- evidence_spans
- [{"source_document": "artifacts/iodine-metabolism-sources/15965468.txt", "locator": "Figure 1E-F; Methods: Constructs and ubiquitination assay", "start_char": 13661, "end_char": 14961}]
- experimental_model
- HEK293 knockdown, recombinant mouse Wsb1/human DIO2 expression and ubiquitination assays; separate chicken explant experiments
- exposure
- Mouse Wsb1 versus BC-box mutant M1; assay contained 30 micromolar ubiquitin and 10 micromolar ubiquitin aldehyde.
- limitations
- Paper uses both wild-type FLAG-D2 and Sec133C/Sec266C constructs; this record concerns ubiquitination machinery, not dietary selenium dependence.
- nutrient_topic
- Iodine research collection; topical membership is not evidence of a direct dietary effect. · Iodine
- organism
- Mouse Wsb1 with human DIO2 constructs; cell-free expression system
- plain_language
- An ubiquitin ligase component marks DIO2 for regulation.
- primary_references
- [i-met-15965468] The Hedgehog-inducible ubiquitin ligase subunit WSB-1 modulates thyroid hormone activation and PTHrP secretion in the developing growth plate. (2005). https://pubmed.ncbi.nlm.nih.gov/15965468/ DOI: 10.1038/ncb1272
- tissue_or_cell_type
- Reconstituted ubiquitination assay
Iodine: thyroid hormone production, deficiency, excess and nutrient interactions (2026-09-17) · lines 1144–1156
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · HEK293 knockdown, recombinant mouse Wsb1/human DIO2 expression and ubiquitination assays; separate chicken explant experiments · source_derived_draft · unverified_draft
### i-met-wsb1-dio2-ubiquitination Recombinant mouse Wsb1 supported ubiquitination of human DIO2 constructs in a reconstituted assay; disruption of the Wsb1 BC box prevented this effect. Condition category: normal nutrient_topic: Iodine research collection; topical membership is not evidence of a direct dietary effect. plain_language: An ubiquitin ligase component marks DIO2 for regulation. organism: Mouse Wsb1 with human DIO2 constructs; cell-free expression system tissue_or_cell_type: Reconstituted ubiquitination assay experimental_model: HEK293 knockdown, recombinant mouse Wsb1/human DIO2 expression and ubiquitination assays; separate chicken explant experiments limitations: Paper uses both wild-type FLAG-D2 and Sec133C/Sec266C constructs; this record concerns ubiquitination machinery, not dietary selenium dependence. exposure: Mouse Wsb1 versus BC-box mutant M1; assay contained 30 micromolar ubiquitin and 10 micromolar ubiquitin aldehyde. evidence_locator: Figure 1E-F; Methods: Constructs and ubiquitination assay evidence_spans: [{"source_document": "artifacts/iodine-metabolism-sources/15965468.txt", "locator": "Figure 1E-F; Methods: Constructs and ubiquitination assay", "start_char": 13661, "end_char": 14961}] [i-met-15965468] The Hedgehog-inducible ubiquitin ligase subunit WSB-1 modulates thyroid hormone activation and PTHrP secretion in the developing growth plate. (2005). https://pubmed.ncbi.nlm.nih.gov/15965468/ DOI: 10.1038/ncb1272
Complete structured claim and evidenceWSB1 knockdown in HEK293 cells increased steady-state D2 approximately fivefold and prolonged its half-life two- to threefold.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- evidence_locator
- Figure 2D-G; Methods: Constructs and Transfections
- evidence_spans
- [{"source_document": "artifacts/iodine-metabolism-sources/15965468.txt", "locator": "Figure 2D-G; Methods: Constructs and Transfections", "start_char": 14434, "end_char": 15884}]
- experimental_model
- HEK293 knockdown, recombinant mouse Wsb1/human DIO2 expression and ubiquitination assays; separate chicken explant experiments
- exposure
- WSB1 RNA interference; activity assays used FLAG-D2, western analyses used FLAG-CysD2; turnover experiments included cycloheximide.
- limitations
- Wild-type and Sec-to-Cys experimental constructs have different roles; no diet was manipulated.
- nutrient_topic
- Iodine research collection; topical membership is not evidence of a direct dietary effect. · Iodine
- organism
- Human HEK293; mouse Wsb1 used in complementation arms
- plain_language
- Reducing the enzyme-removal machinery allows more DIO2 to remain available.
- primary_references
- [i-met-15965468] The Hedgehog-inducible ubiquitin ligase subunit WSB-1 modulates thyroid hormone activation and PTHrP secretion in the developing growth plate. (2005). https://pubmed.ncbi.nlm.nih.gov/15965468/ DOI: 10.1038/ncb1272
- tissue_or_cell_type
- Transfected HEK293 cells
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Iodine: thyroid hormone production, deficiency, excess and nutrient interactions (2026-09-17) · lines 1158–1170
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · HEK293 knockdown, recombinant mouse Wsb1/human DIO2 expression and ubiquitination assays; separate chicken explant experiments · source_derived_draft · unverified_draft
### i-met-wsb1-knockdown-dio2 WSB1 knockdown in HEK293 cells increased steady-state D2 approximately fivefold and prolonged its half-life two- to threefold. Condition category: machinery_impairment nutrient_topic: Iodine research collection; topical membership is not evidence of a direct dietary effect. plain_language: Reducing the enzyme-removal machinery allows more DIO2 to remain available. organism: Human HEK293; mouse Wsb1 used in complementation arms tissue_or_cell_type: Transfected HEK293 cells experimental_model: HEK293 knockdown, recombinant mouse Wsb1/human DIO2 expression and ubiquitination assays; separate chicken explant experiments limitations: Wild-type and Sec-to-Cys experimental constructs have different roles; no diet was manipulated. exposure: WSB1 RNA interference; activity assays used FLAG-D2, western analyses used FLAG-CysD2; turnover experiments included cycloheximide. evidence_locator: Figure 2D-G; Methods: Constructs and Transfections evidence_spans: [{"source_document": "artifacts/iodine-metabolism-sources/15965468.txt", "locator": "Figure 2D-G; Methods: Constructs and Transfections", "start_char": 14434, "end_char": 15884}] [i-met-15965468] The Hedgehog-inducible ubiquitin ligase subunit WSB-1 modulates thyroid hormone activation and PTHrP secretion in the developing growth plate. (2005). https://pubmed.ncbi.nlm.nih.gov/15965468/ DOI: 10.1038/ncb1272
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.