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(2005). https://pubmed.ncbi.nlm.nih.gov/15965468/ DOI: 10.1038/ncb1272","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Transfected HEK293 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"4d399ce9-6b1d-50df-80e9-a2e62abcb751","evidence_kind":"source_excerpt","locator":"Lines 1158-1170","start_line":1158,"end_line":1170,"excerpt":"### i-met-wsb1-knockdown-dio2\nWSB1 knockdown in HEK293 cells increased steady-state D2 approximately fivefold and prolonged its half-life two- to threefold.\nCondition category: machinery_impairment\nnutrient_topic: Iodine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Reducing the enzyme-removal machinery allows more DIO2 to remain available.\norganism: Human HEK293; mouse Wsb1 used in complementation arms\ntissue_or_cell_type: Transfected HEK293 cells\nexperimental_model: HEK293 knockdown, recombinant mouse Wsb1/human DIO2 expression and ubiquitination assays; separate chicken explant experiments\nlimitations: Wild-type and Sec-to-Cys experimental constructs have different roles; no diet was manipulated.\nexposure: WSB1 RNA interference; activity assays used FLAG-D2, western analyses used FLAG-CysD2; turnover experiments included cycloheximide.\nevidence_locator: Figure 2D-G; Methods: Constructs and Transfections\nevidence_spans: [{\"source_document\": \"artifacts/iodine-metabolism-sources/15965468.txt\", \"locator\": \"Figure 2D-G; Methods: Constructs and Transfections\", \"start_char\": 14434, \"end_char\": 15884}]\n[i-met-15965468] The Hedgehog-inducible ubiquitin ligase subunit WSB-1 modulates thyroid hormone activation and PTHrP secretion in the developing growth plate. (2005). https://pubmed.ncbi.nlm.nih.gov/15965468/ DOI: 10.1038/ncb1272","model_system":"HEK293 knockdown, recombinant mouse Wsb1/human DIO2 expression and ubiquitination assays; separate chicken explant experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [i-met-15965468] The Hedgehog-inducible ubiquitin ligase subunit WSB-1 modulates thyroid hormone activation and PTHrP secretion in the developing growth plate. 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