Component
Human histone H4 K12 biotinylation reported in selected assays
Human histone H4 K12 biotinylation reported in selected assays. Species, exposure and limitations are retained in each linked claim.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
The 2004 study identified H4 K8/K12 as candidate biotinylation sites in peptide assays and reported K12-biotinylated H4 immunoreactivity in human nuclear extracts.
Experimental context and source evidence
- evidence_span
- {"source_cache": "artifacts/biotin-research/15153116.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "a3297d283f8a3980a77f1ba1d53f326fb32ee3971c76bda71ef9ba235fb53965", "start_char": 0, "end_char": 1762, "text_sha256": "a3297d283f8a3980a77f1ba1d53f326fb32ee3971c76bda71ef9ba235fb53965"}
- experimental_model
- Synthetic human histone H4 peptide biotinylation and human nuclear-extract immunodetection
- exposure
- Biotinidase incubation, residue substitutions and antibody/streptavidin detection
- limitations
- Peptide reactions and immunoreactivity do not establish abundant endogenous histone biotinylation; later assay-method work explicitly challenges this interpretation.
- nutrient_topic
- Biotin research collection; topical membership is not evidence of a direct dietary effect. · Biotin
- organism
- Homo sapiens
- plain_language
- Early experiments proposed that biotin could also mark histones.
- primary_references
- [b7-p15153116] K8 and K12 are biotinylated in human histone H4. (2004). https://pubmed.ncbi.nlm.nih.gov/15153116/ DOI: 10.1111/j.1432-1033.2004.04167.x
- tissue_or_cell_type
- Synthetic H4 peptides and human cell extracts
Biotin: carboxylases, recycling, deficiency and nutrient interactions (2026-09-17) · lines 1157–1168
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Synthetic human histone H4 peptide biotinylation and human nuclear-extract immunodetection · source_derived_draft · unverified_draft
### b7-histone-h4-report The 2004 study identified H4 K8/K12 as candidate biotinylation sites in peptide assays and reported K12-biotinylated H4 immunoreactivity in human nuclear extracts. Condition category: normal nutrient_topic: Biotin research collection; topical membership is not evidence of a direct dietary effect. plain_language: Early experiments proposed that biotin could also mark histones. organism: Homo sapiens tissue_or_cell_type: Synthetic H4 peptides and human cell extracts experimental_model: Synthetic human histone H4 peptide biotinylation and human nuclear-extract immunodetection limitations: Peptide reactions and immunoreactivity do not establish abundant endogenous histone biotinylation; later assay-method work explicitly challenges this interpretation. exposure: Biotinidase incubation, residue substitutions and antibody/streptavidin detection evidence_span: {"source_cache": "artifacts/biotin-research/15153116.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "a3297d283f8a3980a77f1ba1d53f326fb32ee3971c76bda71ef9ba235fb53965", "start_char": 0, "end_char": 1762, "text_sha256": "a3297d283f8a3980a77f1ba1d53f326fb32ee3971c76bda71ef9ba235fb53965"} [b7-p15153116] K8 and K12 are biotinylated in human histone H4. (2004). https://pubmed.ncbi.nlm.nih.gov/15153116/ DOI: 10.1111/j.1432-1033.2004.04167.x
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.