Component
Cell-surface hERG abundance
Cell-surface hERG abundance; interpretation depends on linked experimental context.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Low extracellular K accelerated hERG internalization and degradation; 0 versus 5 mM exposure strongly reduced mature surface channels.
Experimental context and source evidence
- availability_state
- nutrient_deficiency Imported condition classification; unverified.
- experimental_model
- Transfected HEK293 cells, 6-12-hour low-K exposures.
- limitations
- Zero-K culture is more extreme than ordinary hypokalemia.
- nutrient_topic
- Potassium research collection; topical membership is not evidence of a direct dietary effect. · Potassium
- organism
- Human protein in HEK293 cells
- plain_language
- Potassium outside the cell helped retain a cardiac repolarizing channel.
- primary_references
- [guo-2009-herg] Extracellular K+ concentration controls cell surface density of IKr in rabbit hearts and of the HERG channel in human cell lines (2009). https://www.jci.org/articles/view/39027 DOI: 10.1172/JCI39027
- tissue_or_cell_type
- Heterologous culture
- trigger_kind
- nutrient_deficiency Imported condition classification; unverified.
Potassium: cross-nutrient mechanisms and deficiency (2026-09-17) · lines 693–702
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Transfected HEK293 cells, 6-12-hour low-K exposures. · source_derived_draft · unverified_draft
### k-low-herg-turnover Low extracellular K accelerated hERG internalization and degradation; 0 versus 5 mM exposure strongly reduced mature surface channels. Condition category: nutrient_deficiency nutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect. plain_language: Potassium outside the cell helped retain a cardiac repolarizing channel. organism: Human protein in HEK293 cells tissue_or_cell_type: Heterologous culture experimental_model: Transfected HEK293 cells, 6-12-hour low-K exposures. limitations: Zero-K culture is more extreme than ordinary hypokalemia. [guo-2009-herg] Extracellular K+ concentration controls cell surface density of IKr in rabbit hearts and of the HERG channel in human cell lines (2009). https://www.jci.org/articles/view/39027 DOI: 10.1172/JCI39027
Complete structured claim and evidenceBerberine reduced hERG cell-surface abundance through mechanisms involving caveolin-1 and hERG S6 residues.
Experimental context and source evidence
- evidence_span
- {"source_cache": "artifacts/berberine-research/26543354.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "56eb3a544eb8700c50931e8bbeb16a24786259d3fb9feebb0b2cbe9e81f02734", "start_char": 0, "end_char": 1453, "text_sha256": "56eb3a544eb8700c50931e8bbeb16a24786259d3fb9feebb0b2cbe9e81f02734"}
- experimental_model
- Cell-surface protein regulation and mutagenesis
- exposure
- Berberine exposure and caveolin-1 knockdown
- limitations
- Channel abundance and direct current block are distinct mechanisms. Experimental rescue compounds are not clinical antidote recommendations.
- nutrient_topic
- Berberine research collection; topical membership is not evidence of a direct dietary effect. · Berberine
- organism
- Human hERG-expressing HEK293 cells
- plain_language
- Longer exposure can reduce the number of channels as well as block their current.
- primary_references
- [berberine-p26543354] Mechanism and pharmacological rescue of berberine-induced hERG channel deficiency. (2015). https://pubmed.ncbi.nlm.nih.gov/26543354/ DOI: 10.2147/dddt.s91561
- tissue_or_cell_type
- hERG membrane stability
Berberine: metabolism, nutrient connections and drug interactions (2026-09-17) · lines 623–634
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Cell-surface protein regulation and mutagenesis · source_derived_draft · unverified_draft
### berberine-herg-abundance Berberine reduced hERG cell-surface abundance through mechanisms involving caveolin-1 and hERG S6 residues. Condition category: normal nutrient_topic: Berberine research collection; topical membership is not evidence of a direct dietary effect. plain_language: Longer exposure can reduce the number of channels as well as block their current. organism: Human hERG-expressing HEK293 cells tissue_or_cell_type: hERG membrane stability experimental_model: Cell-surface protein regulation and mutagenesis limitations: Channel abundance and direct current block are distinct mechanisms. Experimental rescue compounds are not clinical antidote recommendations. exposure: Berberine exposure and caveolin-1 knockdown evidence_span: {"source_cache": "artifacts/berberine-research/26543354.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "56eb3a544eb8700c50931e8bbeb16a24786259d3fb9feebb0b2cbe9e81f02734", "start_char": 0, "end_char": 1453, "text_sha256": "56eb3a544eb8700c50931e8bbeb16a24786259d3fb9feebb0b2cbe9e81f02734"} [berberine-p26543354] Mechanism and pharmacological rescue of berberine-induced hERG channel deficiency. (2015). https://pubmed.ncbi.nlm.nih.gov/26543354/ DOI: 10.2147/dddt.s91561
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.