Component
Human GSS L188P, D219A, D219G, Y270C, Y270H and R283C variants
Human GSS L188P, D219A, D219G, Y270C, Y270H and R283C variants. Species, exposure and limitations are retained in each linked claim.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
Six tested GSS variants reduced Vmax to 2–27% of wild-type activity.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- evidence_span
- {"source_cache": "artifacts/glutathione-research/15056072.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "eba1361a80a1f5021b9ca1c841eb5a6f8cc0e79302f68d30a09273debe3f6ee1", "start_char": 0, "end_char": 1151, "text_sha256": "eba1361a80a1f5021b9ca1c841eb5a6f8cc0e79302f68d30a09273debe3f6ee1"}
- experimental_model
- Recombinant patient-associated variant kinetics
- exposure
- Seven missense variants versus wild type
- limitations
- Variants differ; P314L was kinetically neutral in this assay. Clinical severity cannot be read directly from Vmax.
- nutrient_topic
- Glutathione research collection; topical membership is not evidence of a direct dietary effect. · GSH
- organism
- Human GSS expressed in E. coli
- plain_language
- The last assembly step can be the bottleneck.
- primary_references
- [glutathione-p15056072] Human hereditary glutathione synthetase deficiency: kinetic properties of mutant enzymes. (2004). https://pubmed.ncbi.nlm.nih.gov/15056072/ DOI: 10.1042/bj20040114
- tissue_or_cell_type
- Purified enzymes
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Glutathione: metabolism, signaling and nutrient connections (2026-09-17) · lines 411–422
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Recombinant patient-associated variant kinetics · source_derived_draft · unverified_draft
### glutathione-gss-variants Six tested GSS variants reduced Vmax to 2–27% of wild-type activity. Condition category: machinery_impairment nutrient_topic: Glutathione research collection; topical membership is not evidence of a direct dietary effect. plain_language: The last assembly step can be the bottleneck. organism: Human GSS expressed in E. coli tissue_or_cell_type: Purified enzymes experimental_model: Recombinant patient-associated variant kinetics limitations: Variants differ; P314L was kinetically neutral in this assay. Clinical severity cannot be read directly from Vmax. exposure: Seven missense variants versus wild type evidence_span: {"source_cache": "artifacts/glutathione-research/15056072.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "eba1361a80a1f5021b9ca1c841eb5a6f8cc0e79302f68d30a09273debe3f6ee1", "start_char": 0, "end_char": 1151, "text_sha256": "eba1361a80a1f5021b9ca1c841eb5a6f8cc0e79302f68d30a09273debe3f6ee1"} [glutathione-p15056072] Human hereditary glutathione synthetase deficiency: kinetic properties of mutant enzymes. (2004). https://pubmed.ncbi.nlm.nih.gov/15056072/ DOI: 10.1042/bj20040114
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.