Component
GABA-A receptor gamma-2 subunit / GABRG2
GABA-A receptor gamma-2 subunit / GABRG2. Species, exposure and limitations are retained in each linked claim.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
Where it participates (unsigned role)
There were 5, 8 and 13 different GABA-A subunit isoforms identified in human, mouse and rat CD4-positive and CD8-positive T cells respectively, importantly the gamma2 subunit that imposes benzodiazepine sensitivity on the GABA-A receptors was only detected in the mouse T cells, immunoblots and immunocytochemistry showed abundant GABA-A channel proteins in T cells from all three species, GABA-activated whole-cell transient and tonic currents were recorded and were inhibited by picrotoxin, SR95531 and bicuculline, and it is important to bear in mind the interspecies difference when selecting the appropriate animal models and when selecting drugs aimed at modulating human T cell function.
Experimental context and source evidence
- evidence_span
- {"source_cache": "artifacts/gaba-research/22927941.abstract.txt", "locator": "Indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "0457001832d2a3595899415221b7797f5288a787c1d52fd6afa1acf24f032402", "start_char": 0, "end_char": 1589, "text_sha256": "0457001832d2a3595899415221b7797f5288a787c1d52fd6afa1acf24f032402"}
- experimental_model
- Subunit isoform survey with immunoblotting, immunocytochemistry and whole-cell recording across three species
- exposure
- GABA-activated transient and tonic currents, with picrotoxin, SR95531 and bicuculline
- limitations
- The species comparison is the point. Subunit repertoires differ enough that a drug result in one species does not carry to another.
- nutrient_topic
- GABA research collection; topical membership is not evidence of a direct clinical effect, and the sign of a GABA response depends on the chloride gradient of the cell it was measured in. · Gamma-aminobutyric acid
- organism
- Human, mouse and rat
- plain_language
- The subunit that makes a receptor respond to benzodiazepines was found on mouse T cells and not on human ones.
- primary_references
- [gb-p22927941] Different subtypes of GABA-A receptors are expressed in human, mouse and rat T lymphocytes. (2012). https://pubmed.ncbi.nlm.nih.gov/22927941/ DOI: 10.1371/journal.pone.0042959
- tissue_or_cell_type
- CD4 and CD8 T lymphocytes
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Subunit isoform survey with immunoblotting, immunocytochemistry and whole-cell recording across three species · source_derived_draft · unverified_draft
### gb-the-subunits-differ-by-species There were 5, 8 and 13 different GABA-A subunit isoforms identified in human, mouse and rat CD4-positive and CD8-positive T cells respectively, importantly the gamma2 subunit that imposes benzodiazepine sensitivity on the GABA-A receptors was only detected in the mouse T cells, immunoblots and immunocytochemistry showed abundant GABA-A channel proteins in T cells from all three species, GABA-activated whole-cell transient and tonic currents were recorded and were inhibited by picrotoxin, SR95531 and bicuculline, and it is important to bear in mind the interspecies difference when selecting the appropriate animal models and when selecting drugs aimed at modulating human T cell function. Condition category: normal nutrient_topic: GABA research collection; topical membership is not evidence of a direct clinical effect, and the sign of a GABA response depends on the chloride gradient of the cell it was measured in. plain_language: The subunit that makes a receptor respond to benzodiazepines was found on mouse T cells and not on human ones. organism: Human, mouse and rat tissue_or_cell_type: CD4 and CD8 T lymphocytes experimental_model: Subunit isoform survey with immunoblotting, immunocytochemistry and whole-cell recording across three species limitations: The species comparison is the point. Subunit repertoires differ enough that a drug result in one species does not carry to another. exposure: GABA-activated transient and tonic currents, with picrotoxin, SR95531 and bicuculline evidence_span: {"source_cache": "artifacts/gaba-research/22927941.abstract.txt", "locator": "Indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "0457001832d2a3595899415221b7797f5288a787c1d52fd6afa1acf24f032402", "start_char": 0, "end_char": 1589, "text_sha256": "0457001832d2a3595899415221b7797f5288a787c1d52fd6afa1acf24f032402"} [gb-p22927941] Different subtypes of GABA-A receptors are expressed in human, mouse and rat T lymphocytes. (2012). https://pubmed.ncbi.nlm.nih.gov/22927941/ DOI: 10.1371/journal.pone.0042959
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.