Component

Net ammonia generation in human fecal cultures

Net ammonia generation in human fecal cultures. Species, exposure and limitations are retained in each linked claim.

1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. Pectin significantly reduced net ammonia generation in incubations after subjects had been pre-fed pectin; the reduction without pre-feeding was not significant.

    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/pectin-research/2317475.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "86aae760cc52aeca6f270420e20dabaffa8167770fa30c7fa8e35b1f67b7d9b4", "start_char": 0, "end_char": 1642, "text_sha256": "86aae760cc52aeca6f270420e20dabaffa8167770fa30c7fa8e35b1f67b7d9b4"}
    experimental_model
    Anaerobic human fecal incubations before and after carbohydrate pre-feeding
    exposure
    Pectin and comparator carbohydrates; 48-hour incubations, up to two weeks of pre-feeding
    limitations
    Cultures lack intestinal absorption and host metabolism; results do not quantify systemic exposure.
    nutrient_topic
    Pectin research collection; topical membership is not evidence of a direct dietary effect. · Pectin, structurally heterogeneous plant polysaccharides
    organism
    Human fecal microbiota ex vivo
    plain_language
    Previous exposure changed the measured nitrogen-metabolism response.
    primary_references
    [pectin-p2317475] The effect of lactulose, pectin, arabinogalactan and cellulose on the production of organic acids and metabolism of ammonia by intestinal bacteria in a faecal incubation system. (1990). https://pubmed.ncbi.nlm.nih.gov/2317475/ DOI: 10.1079/bjn19900088
    tissue_or_cell_type
    Batch cultures

    Pectin: metabolism, signaling and nutrient connections (2026-09-17) · lines 880–891

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Anaerobic human fecal incubations before and after carbohydrate pre-feeding · source_derived_draft · unverified_draft

    ### pectin-ammonia Pectin significantly reduced net ammonia generation in incubations after subjects had been pre-fed pectin; the reduction without pre-feeding was not significant. Condition category: normal nutrient_topic: Pectin research collection; topical membership is not evidence of a direct dietary effect. plain_language: Previous exposure changed the measured nitrogen-metabolism response. organism: Human fecal microbiota ex vivo tissue_or_cell_type: Batch cultures experimental_model: Anaerobic human fecal incubations before and after carbohydrate pre-feeding limitations: Cultures lack intestinal absorption and host metabolism; results do not quantify systemic exposure. exposure: Pectin and comparator carbohydrates; 48-hour incubations, up to two weeks of pre-feeding evidence_span: {"source_cache": "artifacts/pectin-research/2317475.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "86aae760cc52aeca6f270420e20dabaffa8167770fa30c7fa8e35b1f67b7d9b4", "start_char": 0, "end_char": 1642, "text_sha256": "86aae760cc52aeca6f270420e20dabaffa8167770fa30c7fa8e35b1f67b7d9b4"} [pectin-p2317475] The effect of lactulose, pectin, arabinogalactan and cellulose on the production of organic acids and metabolism of ammonia by intestinal bacteria in a faecal incubation system. (1990). https://pubmed.ncbi.nlm.nih.gov/2317475/ DOI: 10.1079/bjn19900088
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards