Component
E. coli growth dependent on external aromatic amino acids
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Loss of both AroK and AroL produces aromatic-amino-acid auxotrophy in E. coli.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- evidence_access
- Primary full-text HTML and indexed abstract reviewed for the stated results; archived locally. Primary publisher article introductory text reviewed; precise affinity estimates are contextual, not newly remeasured.
- experimental_condition
- Functional shikimate kinase background lost · Escherichia coli shikimate kinase I / AroK Condition belongs to the full experimental contrast; do not separate a joint intervention.
- experimental_condition
- Functional shikimate kinase background lost · Escherichia coli shikimate kinase II / AroL Condition belongs to the full experimental contrast; do not separate a joint intervention.
- experimental_contrast
- {"intervention": "Both AroK and AroL unavailable", "comparator": "Functional shikimate kinase background", "endpoint": "Loss of both AroK and AroL produces aromatic-amino-acid auxotrophy in E. coli.", "effect_direction": "increase", "combination": "joint", "conditions": [{"entity_slug": "ecoli-arok", "state": "lost"}, {"entity_slug": "ecoli-arol", "state": "lost"}]} Explicit extracted experimental comparison; source-derived draft.
- experimental_model
- E. coli double-kinase loss phenotype discussed in the primary structural study.
- interpretation_status
- Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.
- limitations
- Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.
- plain_language
- Loss of both AroK and AroL produces aromatic-amino-acid auxotrophy in E. coli.
- primary_references
- Biochemical and X-ray crystallographic studies on shikimate kinase: the important structural role of the P-loop lysine. | 2001 | DOI 10.1110/ps.52501 | PMID 11369852 | https://pubmed.ncbi.nlm.nih.gov/11369852/ | https://doi.org/10.1110/ps.52501 | https://pmc.ncbi.nlm.nih.gov/articles/PMC2374015/
- primary_references
- Crystal structure of the Escherichia coli shikimate kinase I (AroK) that confers sensitivity to mecillinam. | 2002 | DOI 10.1002/prot.10099 | PMID 12001235 | https://pubmed.ncbi.nlm.nih.gov/12001235/ | https://doi.org/10.1002/prot.10099
- source_locator
- Reviewed reference lines 15-15; exact primary location described in quoted passage where extracted.
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Shikimic acid: detailed mechanisms of action (reviewed 5 October 2026) · lines 15–15
Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · E. coli double-kinase loss phenotype discussed in the primary structural study. · Recorded from source · Recorded from source
**ATP and magnesium at shikimate kinase.** Shikimate kinase transfers a phosphoryl group from ATP to shikimate, yielding shikimate-3-phosphate and ADP. The Erwinia chrysanthemi enzyme study identifies magnesium dependence and a Mg–ADP structural complex; this is a microbial enzyme requirement, not a demonstrated human magnesium-deficiency phenotype. E. coli has AroK and AroL kinase isoenzymes with different substrate affinities. The AroK structural paper discusses approximate shikimate Km values of 20 mM versus 0.2 mM for the two systems; these are organism/assay properties, not plasma targets. Loss of both kinases produces aromatic-amino-acid auxotrophy; environmental nutrient rescue and isoenzyme compensation matter. [Biochemical and X-ray crystallographic studies on shikimate kinase: the important structural role of the P-loop lysine.](https://pubmed.ncbi.nlm.nih.gov/11369852/) [Crystal structure of the Escherichia coli shikimate kinase I (AroK) that confers sensitivity to mecillinam.](https://pubmed.ncbi.nlm.nih.gov/12001235/)
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.