Component
Escherichia coli BW25113 tryptophanase / TnaA
Context-specific entity; species, compartment and exposure are stated on each claim.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
TnaA also converted 5-HTP to 5-hydroxyindole, with lower catalytic efficiency than for tryptophan.
Experimental context and source evidence
- evidence_access
- Primary full text, Figure 2
- experimental_model
- Purified E. coli enzyme and tnaA-mutant experiments.
- limitations
- 5-HTP and tryptophan are distinct substrates, not interchangeable supplement exposures.
- nutrient_topic
- Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit. · L-Tryptophan
- plain_language
- The serotonin precursor can instead become a microbial product.
- primary_references
- Gut bacteria-derived 5-hydroxyindole is a potent stimulant of intestinal motility via its action on L-type calcium channels. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33481771/ · DOI 10.1371/journal.pbio.3001070
Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19) · lines 642–648
AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text. · supports · Purified E. coli enzyme and tnaA-mutant experiments. · source_derived_draft · unverified_draft
## tryptophan-tnaa-hydroxyindole The serotonin precursor can instead become a microbial product. TnaA also converted 5-HTP to 5-hydroxyindole, with lower catalytic efficiency than for tryptophan. Model: Purified E. coli enzyme and tnaA-mutant experiments. Limitations: 5-HTP and tryptophan are distinct substrates, not interchangeable supplement exposures. Evidence access: Primary full text, Figure 2 Gut bacteria-derived 5-hydroxyindole is a potent stimulant of intestinal motility via its action on L-type calcium channels. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33481771/ · DOI 10.1371/journal.pbio.3001070
Complete structured claim and evidencePurified E. coli BW25113 TnaA converted L-tryptophan to indole in a PLP-containing assay; tryptophan Km was 0.19 ± 0.009 mM.
Experimental context and source evidence
- evidence_access
- Primary full text, Figure 2 and enzyme-kinetics methods
- experimental_model
- Purified bacterial enzyme kinetics.
- limitations
- Culture kinetics do not predict a person’s production rate.
- nutrient_topic
- Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit. · L-Tryptophan
- plain_language
- A bacterial enzyme creates the indole that can enter host liver metabolism.
- primary_references
- Gut bacteria-derived 5-hydroxyindole is a potent stimulant of intestinal motility via its action on L-type calcium channels. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33481771/ · DOI 10.1371/journal.pbio.3001070
Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19) · lines 634–640
AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text. · supports · Purified bacterial enzyme kinetics. · source_derived_draft · unverified_draft
## tryptophan-tnaa-indole A bacterial enzyme creates the indole that can enter host liver metabolism. Purified E. coli BW25113 TnaA converted L-tryptophan to indole in a PLP-containing assay; tryptophan Km was 0.19 ± 0.009 mM. Model: Purified bacterial enzyme kinetics. Limitations: Culture kinetics do not predict a person’s production rate. Evidence access: Primary full text, Figure 2 and enzyme-kinetics methods Gut bacteria-derived 5-hydroxyindole is a potent stimulant of intestinal motility via its action on L-type calcium channels. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33481771/ · DOI 10.1371/journal.pbio.3001070
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.