Component
1,2-Dihydroxyethyl-ThDP intermediate
ThDP-bound two-carbon donor intermediate of transketolase; distinct from the one-hydroxyl PDH intermediate.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Acid-quench NMR of human TKT identified the protonated form of the DHE-ThDP intermediate generated during the donor half-reaction.
Experimental context and source evidence
- evidence
- [{"paper_key": "mitschke-2010-tkt", "source_bundle": "artifacts/thiamine_metabolism_sources.json", "passage_ids": ["p-31", "p-44", "p-46", "p-47"], "locator": "acid quench", "preservation": "Exact text retained in the source bundle; full source document retained when openly retrievable."}]
- experimental_model
- Human recombinant TKT; chemical quench and NMR.
- limitations
- Acid quench protonates the intermediate; native enamine occupancy is inferred from trapped species.
- nutrient
- Thiamine (vitamin B1) · Thiamine (vitamin B1)
- nutrient_topic
- Thiamine research collection; topical membership is not evidence of a direct dietary effect. · Thiamine (vitamin B1)
- organism
- Homo sapiens
- plain_language
- B1 temporarily carries the two-carbon sugar fragment before its transfer to an acceptor; the detected acid-trapped form is distinguished from the reactive enamine.
- primary_references
- [mitschke-2010-tkt] The crystal structure of human transketolase and new insights into its mode of action (2010). https://pubmed.ncbi.nlm.nih.gov/20667822/ DOI: 10.1074/jbc.m110.149955
- tissue_or_cell_type
- Purified enzyme
Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 963–974
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human recombinant TKT; chemical quench and NMR. · source_derived_draft · unverified_draft
### b1-tkt-dhe-thdp-intermediate Acid-quench NMR of human TKT identified the protonated form of the DHE-ThDP intermediate generated during the donor half-reaction. Condition category: normal nutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect. plain_language: B1 temporarily carries the two-carbon sugar fragment before its transfer to an acceptor; the detected acid-trapped form is distinguished from the reactive enamine. organism: Homo sapiens tissue_or_cell_type: Purified enzyme experimental_model: Human recombinant TKT; chemical quench and NMR. limitations: Acid quench protonates the intermediate; native enamine occupancy is inferred from trapped species. evidence: [{"paper_key": "mitschke-2010-tkt", "source_bundle": "artifacts/thiamine_metabolism_sources.json", "passage_ids": ["p-31", "p-44", "p-46", "p-47"], "locator": "acid quench", "preservation": "Exact text retained in the source bundle; full source document retained when openly retrievable."}] nutrient: Thiamine (vitamin B1) [mitschke-2010-tkt] The crystal structure of human transketolase and new insights into its mode of action (2010). https://pubmed.ncbi.nlm.nih.gov/20667822/ DOI: 10.1074/jbc.m110.149955
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.