Human ClC-7 chloride/proton exchanger / CLCN7: molecular mechanisms & dependencies

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

Human evidence only

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

Where it participates (unsigned role)

Tail-current reversal potentials supported two chloride ions exchanged per proton by ClC-7/Ostm1.
Engineered plasma-membrane ClC-7/Ostm1 assay complexes → Chloride ion source_derived_draftungraded
Experimental context and source evidence
evidence_span
{"source_cache": "artifacts/chloride-research/21527911.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "1a83cfa67615e467858b2a56c2e6b6e1ae8414a28c553b8a645d7789987f53c5", "start_char": 0, "end_char": 1081, "text_sha256": "1a83cfa67615e467858b2a56c2e6b6e1ae8414a28c553b8a645d7789987f53c5"}
experimental_model
Electrophysiology of plasma-membrane-targeted constructs
exposure
ClC-7 sorting-motif mutations and Ostm1 constructs
limitations
Engineering enabled measurement outside lysosomes. Species checked in author manuscript https://edoc.mdc-berlin.de/id/eprint/11621/1/11621oa.pdf, Results; native direction depends on gradients.
nutrient_topic
Chloride research collection; topical membership is not evidence of a direct dietary effect. · Chloride
organism
Human and rat ClC-7; Xenopus oocytes and mammalian cells
plain_language
The bone-related chloride transporter is a coupled exchanger, not a simple chloride pore.
primary_references
[chloride-p21527911] ClC-7 is a slowly voltage-gated 2Cl(-)/1H(+)-exchanger and requires Ostm1 for transport activity. (2011). https://pubmed.ncbi.nlm.nih.gov/21527911/ DOI: 10.1038/emboj.2011.137
tissue_or_cell_type
Engineered plasma membrane
Chloride: transport, acid-base balance, nutrient interactions and loss states (2026-09-17) · lines 744–755
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Electrophysiology of plasma-membrane-targeted constructs · source_derived_draft · unverified_draft
### chloride-clc7-stoichiometry Tail-current reversal potentials supported two chloride ions exchanged per proton by ClC-7/Ostm1. Condition category: normal nutrient_topic: Chloride research collection; topical membership is not evidence of a direct dietary effect. plain_language: The bone-related chloride transporter is a coupled exchanger, not a simple chloride pore. organism: Human and rat ClC-7; Xenopus oocytes and mammalian cells tissue_or_cell_type: Engineered plasma membrane experimental_model: Electrophysiology of plasma-membrane-targeted constructs limitations: Engineering enabled measurement outside lysosomes. Species checked in author manuscript https://edoc.mdc-berlin.de/id/eprint/11621/1/11621oa.pdf, Results; native direction depends on gradients. exposure: ClC-7 sorting-motif mutations and Ostm1 constructs evidence_span: {"source_cache": "artifacts/chloride-research/21527911.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "1a83cfa67615e467858b2a56c2e6b6e1ae8414a28c553b8a645d7789987f53c5", "start_char": 0, "end_char": 1081, "text_sha256": "1a83cfa67615e467858b2a56c2e6b6e1ae8414a28c553b8a645d7789987f53c5"} [chloride-p21527911] ClC-7 is a slowly voltage-gated 2Cl(-)/1H(+)-exchanger and requires Ostm1 for transport activity. (2011). https://pubmed.ncbi.nlm.nih.gov/21527911/ DOI: 10.1038/emboj.2011.137
Complete structured claim and evidence
Both the amino-terminal and transmembrane regions of Ostm1 were required for the measured ClC-7 exchange activity.
Mammalian OSTM1 ClC-7 accessory subunit → Engineered plasma-membrane ClC-7/Ostm1 assay complexes source_derived_draftungraded
Experimental context and source evidence
evidence_span
{"source_cache": "artifacts/chloride-research/21527911.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "1a83cfa67615e467858b2a56c2e6b6e1ae8414a28c553b8a645d7789987f53c5", "start_char": 0, "end_char": 1081, "text_sha256": "1a83cfa67615e467858b2a56c2e6b6e1ae8414a28c553b8a645d7789987f53c5"}
experimental_model
Electrophysiology of plasma-membrane-targeted constructs
exposure
ClC-7 sorting-motif mutations and Ostm1 constructs
limitations
Engineering enabled measurement outside lysosomes. Species checked in author manuscript https://edoc.mdc-berlin.de/id/eprint/11621/1/11621oa.pdf, Results; native direction depends on gradients.
nutrient_topic
Chloride research collection; topical membership is not evidence of a direct dietary effect. · Chloride
organism
Human and rat ClC-7; Xenopus oocytes and mammalian cells
plain_language
A separate accessory protein is needed for the exchanger to work.
primary_references
[chloride-p21527911] ClC-7 is a slowly voltage-gated 2Cl(-)/1H(+)-exchanger and requires Ostm1 for transport activity. (2011). https://pubmed.ncbi.nlm.nih.gov/21527911/ DOI: 10.1038/emboj.2011.137
tissue_or_cell_type
Engineered plasma membrane
Chloride: transport, acid-base balance, nutrient interactions and loss states (2026-09-17) · lines 757–768
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Electrophysiology of plasma-membrane-targeted constructs · source_derived_draft · unverified_draft
### chloride-ostm1-clc7 Both the amino-terminal and transmembrane regions of Ostm1 were required for the measured ClC-7 exchange activity. Condition category: normal nutrient_topic: Chloride research collection; topical membership is not evidence of a direct dietary effect. plain_language: A separate accessory protein is needed for the exchanger to work. organism: Human and rat ClC-7; Xenopus oocytes and mammalian cells tissue_or_cell_type: Engineered plasma membrane experimental_model: Electrophysiology of plasma-membrane-targeted constructs limitations: Engineering enabled measurement outside lysosomes. Species checked in author manuscript https://edoc.mdc-berlin.de/id/eprint/11621/1/11621oa.pdf, Results; native direction depends on gradients. exposure: ClC-7 sorting-motif mutations and Ostm1 constructs evidence_span: {"source_cache": "artifacts/chloride-research/21527911.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "1a83cfa67615e467858b2a56c2e6b6e1ae8414a28c553b8a645d7789987f53c5", "start_char": 0, "end_char": 1081, "text_sha256": "1a83cfa67615e467858b2a56c2e6b6e1ae8414a28c553b8a645d7789987f53c5"} [chloride-p21527911] ClC-7 is a slowly voltage-gated 2Cl(-)/1H(+)-exchanger and requires Ostm1 for transport activity. (2011). https://pubmed.ncbi.nlm.nih.gov/21527911/ DOI: 10.1038/emboj.2011.137
Complete structured claim and evidence

The events it takes part in

A mechanism often involves more than two components. These are the full events, with every participant and its role.

Situations it appears in

Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

Open hypotheses

Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.

This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

Human ClC-7 chloride/proton exchanger / CLCN7