{"id":"81db426a-eb11-5201-9d50-d2f5327a17b6","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-ostm1-clc7","predicate":"required_for_activity_of","statement":"Both the amino-terminal and transmembrane regions of Ostm1 were required for the measured ClC-7 exchange activity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"93d1a8d3-640e-5e4d-8488-d1ac8f2042c6","mechanism_event_label":"A separate accessory protein is needed for the exchanger to work.","subject":{"id":"fbc9179c-2df3-555a-8df1-080d4a33fc13","slug":"ostm1","display_name":"Mammalian OSTM1 ClC-7 accessory subunit","entity_type_key":"protein"},"object":{"id":"000de00e-854c-5dcd-99f7-5be5ce6ba43d","slug":"clc7-pm-ostm1","display_name":"Engineered plasma-membrane ClC-7/Ostm1 assay complexes","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"93d1a8d3-640e-5e4d-8488-d1ac8f2042c6","stable_key":"4df6a932-097e-563a-91fe-5dceec471ffd:chloride-ostm1-clc7-event","event_type":"biochemical_relationship","label":"A separate accessory protein is needed for the exchanger to work.","description":"Both the amino-terminal and transmembrane regions of Ostm1 were required for the measured ClC-7 exchange activity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f417881f-8a59-5514-995d-c33f98cee54e","slug":"clcn7","display_name":"Human ClC-7 chloride/proton exchanger / CLCN7","entity_type_key":"protein"},"role":"human_construct_parent","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9a9b1617-2600-5a8c-8711-375e2564895a","slug":"rat-clcn7","display_name":"Rat ClC-7 chloride/proton exchanger / Clcn7","entity_type_key":"protein"},"role":"rat_construct_parent","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fbc9179c-2df3-555a-8df1-080d4a33fc13","slug":"ostm1","display_name":"Mammalian OSTM1 ClC-7 accessory subunit","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"000de00e-854c-5dcd-99f7-5be5ce6ba43d","slug":"clc7-pm-ostm1","display_name":"Engineered plasma-membrane ClC-7/Ostm1 assay complexes","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chloride-research/21527911.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"1a83cfa67615e467858b2a56c2e6b6e1ae8414a28c553b8a645d7789987f53c5\", \"start_char\": 0, \"end_char\": 1081, \"text_sha256\": \"1a83cfa67615e467858b2a56c2e6b6e1ae8414a28c553b8a645d7789987f53c5\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Electrophysiology of plasma-membrane-targeted constructs","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"ClC-7 sorting-motif mutations and Ostm1 constructs","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Engineering enabled measurement outside lysosomes. 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(2011). https://pubmed.ncbi.nlm.nih.gov/21527911/ DOI: 10.1038/emboj.2011.137","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Engineered plasma membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8197e2d8-f881-533a-9efa-48799a5361b2","evidence_kind":"source_excerpt","locator":"Lines 757-768","start_line":757,"end_line":768,"excerpt":"### chloride-ostm1-clc7\nBoth the amino-terminal and transmembrane regions of Ostm1 were required for the measured ClC-7 exchange activity.\nCondition category: normal\nnutrient_topic: Chloride research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A separate accessory protein is needed for the exchanger to work.\norganism: Human and rat ClC-7; Xenopus oocytes and mammalian cells\ntissue_or_cell_type: Engineered plasma membrane\nexperimental_model: Electrophysiology of plasma-membrane-targeted constructs\nlimitations: Engineering enabled measurement outside lysosomes. Species checked in author manuscript https://edoc.mdc-berlin.de/id/eprint/11621/1/11621oa.pdf, Results; native direction depends on gradients.\nexposure: ClC-7 sorting-motif mutations and Ostm1 constructs\nevidence_span: {\"source_cache\": \"artifacts/chloride-research/21527911.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"1a83cfa67615e467858b2a56c2e6b6e1ae8414a28c553b8a645d7789987f53c5\", \"start_char\": 0, \"end_char\": 1081, \"text_sha256\": \"1a83cfa67615e467858b2a56c2e6b6e1ae8414a28c553b8a645d7789987f53c5\"}\n[chloride-p21527911] ClC-7 is a slowly voltage-gated 2Cl(-)/1H(+)-exchanger and requires Ostm1 for transport activity. 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