Component
CaV1.2-mediated ionic current
Current through CaV1.2; permeant calcium, barium or sodium is specified per experiment.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
CaV1.2 EF-hand substitutions shifted Mg inhibition, with D1546K eliminating sensitivity across the tested range.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- cross_nutrient
- The regulatory protein site connects intracellular magnesium with calcium-channel current.
- evidence-system
- EF-hand mutagenesis and patch clamp; rabbit cardiac CaV1.2 with beta1b and alpha2delta auxiliary subunits
- experimental_model
- EF-hand mutagenesis and patch clamp; rabbit cardiac CaV1.2 with beta1b and alpha2delta auxiliary subunits
- exposure
- Calculated intracellular free Mg was varied over 0.1-7.2 mM, with a 0.8 mM control; pipette MgATP was held at 5 mM. Ca2+ or Ba2+ served as the permeant ion in separate recordings.
- limitations
- Supports an EF-hand-associated regulatory mechanism; not direct structural observation of bound Mg.
- nutrient_topic
- Magnesium research collection; topical membership is not evidence of a direct dietary effect. · Magnesium
- organism
- Rabbit cardiac CaV1.2 protein expressed in human-derived tsA-201 cells
- plain_language
- Changing the channel regulatory site changed its response to intracellular magnesium.
- primary_references
- [brunet-2005-cav12] Modulation of CaV1.2 channels by Mg2+ acting at an EF-hand motif in the COOH-terminal domain (2005). https://pmc.ncbi.nlm.nih.gov/articles/PMC2266622/ DOI: 10.1085/jgp.200509333
- tissue
- tsA-201 cells
- tissue_or_cell_type
- tsA-201 cells
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17) · lines 1303–1316
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · EF-hand mutagenesis and patch clamp; rabbit cardiac CaV1.2 with beta1b and alpha2delta auxiliary subunits · source_derived_draft · unverified_draft
### cav12-ef-hand-magnesium-sensitivity CaV1.2 EF-hand substitutions shifted Mg inhibition, with D1546K eliminating sensitivity across the tested range. Condition category: machinery_impairment nutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect. plain_language: Changing the channel regulatory site changed its response to intracellular magnesium. organism: Rabbit cardiac CaV1.2 protein expressed in human-derived tsA-201 cells tissue_or_cell_type: tsA-201 cells experimental_model: EF-hand mutagenesis and patch clamp; rabbit cardiac CaV1.2 with beta1b and alpha2delta auxiliary subunits limitations: Supports an EF-hand-associated regulatory mechanism; not direct structural observation of bound Mg. cross_nutrient: The regulatory protein site connects intracellular magnesium with calcium-channel current. exposure: Calculated intracellular free Mg was varied over 0.1-7.2 mM, with a 0.8 mM control; pipette MgATP was held at 5 mM. Ca2+ or Ba2+ served as the permeant ion in separate recordings. evidence-system: EF-hand mutagenesis and patch clamp; rabbit cardiac CaV1.2 with beta1b and alpha2delta auxiliary subunits tissue: tsA-201 cells [brunet-2005-cav12] Modulation of CaV1.2 channels by Mg2+ acting at an EF-hand motif in the COOH-terminal domain (2005). https://pmc.ncbi.nlm.nih.gov/articles/PMC2266622/ DOI: 10.1085/jgp.200509333
Complete structured claim and evidenceIncreasing intracellular free Mg reduced Ca2+ and Ba2+ currents through recombinant CaV1.2.
Experimental context and source evidence
- cross_nutrient
- Mg availability directly modulated Ca-channel function in an expression assay, not intestinal calcium absorption.
- evidence-system
- Whole-cell recordings with intracellular free-Mg control; rabbit cardiac CaV1.2 with beta1b and alpha2delta auxiliary subunits
- experimental_model
- Whole-cell recordings with intracellular free-Mg control; rabbit cardiac CaV1.2 with beta1b and alpha2delta auxiliary subunits
- exposure
- Calculated intracellular free Mg was varied over 0.1-7.2 mM, with a 0.8 mM control; pipette MgATP was held at 5 mM. Ca2+ or Ba2+ served as the permeant ion in separate recordings.
- limitations
- Free Mg was experimentally controlled; no dietary intake, serum threshold or cardiac event was measured.
- nutrient_topic
- Magnesium research collection; topical membership is not evidence of a direct dietary effect. · Magnesium
- organism
- Rabbit cardiac CaV1.2 protein expressed in human-derived tsA-201 cells
- plain_language
- Free magnesium inside the cell can restrain current through a calcium-entry channel.
- primary_references
- [brunet-2005-cav12] Modulation of CaV1.2 channels by Mg2+ acting at an EF-hand motif in the COOH-terminal domain (2005). https://pmc.ncbi.nlm.nih.gov/articles/PMC2266622/ DOI: 10.1085/jgp.200509333
- tissue
- tsA-201 cells
- tissue_or_cell_type
- tsA-201 cells
Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17) · lines 1288–1301
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Whole-cell recordings with intracellular free-Mg control; rabbit cardiac CaV1.2 with beta1b and alpha2delta auxiliary subunits · source_derived_draft · unverified_draft
### intracellular-magnesium-reduces-cav12-current Increasing intracellular free Mg reduced Ca2+ and Ba2+ currents through recombinant CaV1.2. Condition category: normal nutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect. plain_language: Free magnesium inside the cell can restrain current through a calcium-entry channel. organism: Rabbit cardiac CaV1.2 protein expressed in human-derived tsA-201 cells tissue_or_cell_type: tsA-201 cells experimental_model: Whole-cell recordings with intracellular free-Mg control; rabbit cardiac CaV1.2 with beta1b and alpha2delta auxiliary subunits limitations: Free Mg was experimentally controlled; no dietary intake, serum threshold or cardiac event was measured. cross_nutrient: Mg availability directly modulated Ca-channel function in an expression assay, not intestinal calcium absorption. exposure: Calculated intracellular free Mg was varied over 0.1-7.2 mM, with a 0.8 mM control; pipette MgATP was held at 5 mM. Ca2+ or Ba2+ served as the permeant ion in separate recordings. evidence-system: Whole-cell recordings with intracellular free-Mg control; rabbit cardiac CaV1.2 with beta1b and alpha2delta auxiliary subunits tissue: tsA-201 cells [brunet-2005-cav12] Modulation of CaV1.2 channels by Mg2+ acting at an EF-hand motif in the COOH-terminal domain (2005). https://pmc.ncbi.nlm.nih.gov/articles/PMC2266622/ DOI: 10.1085/jgp.200509333
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.