Component
The alpha-2-delta subunit of a voltage-dependent calcium channel
The alpha-2-delta subunit of a voltage-dependent calcium channel. Species, exposure and limitations are retained in each linked claim.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
Gabapentin is a novel anticonvulsant drug with a mechanism of action apparently dissimilar to that of other antiepileptic agents, the detergent-solubilized binding protein from pig cerebral cortex membranes was purified 1022-fold, the purified protein had an apparent subunit molecular weight of 130,000 and was heavily glycosylated, its partial N-terminal amino acid sequence EPFPSAVTIK was identical to that reported for the alpha2delta subunit of the L-type calcium channel, binding to cells transfected with alpha2delta complementary DNA was elevated more than 10-fold over controls, and tritiated gabapentin is the first pharmacological agent described that interacts with an alpha2delta subunit of a voltage-dependent calcium channel.
Experimental context and source evidence
- evidence_span
- {"source_cache": "artifacts/gaba-research/8621444.abstract.txt", "locator": "Indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "1723620d459b4f48ad397d59284df3f0619894ba82c6d066a42a47a0edb22216", "start_char": 0, "end_char": 1531, "text_sha256": "1723620d459b4f48ad397d59284df3f0619894ba82c6d066a42a47a0edb22216"}
- experimental_model
- Purification of a radioligand binding protein from pig cerebral cortex with N-terminal sequencing and expression in transfected cells
- exposure
- Tritiated gabapentin binding, followed through a six-step purification to protein identity
- limitations
- A binding and purification study. It identifies what the drug binds; it does not test the GABA receptors, so the absence of GABA-receptor binding is not measured here.
- nutrient_topic
- GABA research collection; topical membership is not evidence of a direct clinical effect, and the sign of a GABA response depends on the chloride gradient of the cell it was measured in. · Gamma-aminobutyric acid
- organism
- Pig and rat
- plain_language
- The anticonvulsant designed as a GABA analogue turned out to bind a calcium channel subunit instead.
- primary_references
- [gb-p8621444] The novel anticonvulsant drug, gabapentin (Neurontin), binds to the alpha2delta subunit of a calcium channel. (1996). https://pubmed.ncbi.nlm.nih.gov/8621444/ DOI: 10.1074/jbc.271.10.5768
- tissue_or_cell_type
- Cerebral cortex, heart and skeletal muscle
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Purification of a radioligand binding protein from pig cerebral cortex with N-terminal sequencing and expression in transfected cells · source_derived_draft · unverified_draft
### gb-the-drug-named-for-gaba-binds-a-calcium-channel Gabapentin is a novel anticonvulsant drug with a mechanism of action apparently dissimilar to that of other antiepileptic agents, the detergent-solubilized binding protein from pig cerebral cortex membranes was purified 1022-fold, the purified protein had an apparent subunit molecular weight of 130,000 and was heavily glycosylated, its partial N-terminal amino acid sequence EPFPSAVTIK was identical to that reported for the alpha2delta subunit of the L-type calcium channel, binding to cells transfected with alpha2delta complementary DNA was elevated more than 10-fold over controls, and tritiated gabapentin is the first pharmacological agent described that interacts with an alpha2delta subunit of a voltage-dependent calcium channel. Condition category: normal nutrient_topic: GABA research collection; topical membership is not evidence of a direct clinical effect, and the sign of a GABA response depends on the chloride gradient of the cell it was measured in. plain_language: The anticonvulsant designed as a GABA analogue turned out to bind a calcium channel subunit instead. organism: Pig and rat tissue_or_cell_type: Cerebral cortex, heart and skeletal muscle experimental_model: Purification of a radioligand binding protein from pig cerebral cortex with N-terminal sequencing and expression in transfected cells limitations: A binding and purification study. It identifies what the drug binds; it does not test the GABA receptors, so the absence of GABA-receptor binding is not measured here. exposure: Tritiated gabapentin binding, followed through a six-step purification to protein identity evidence_span: {"source_cache": "artifacts/gaba-research/8621444.abstract.txt", "locator": "Indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "1723620d459b4f48ad397d59284df3f0619894ba82c6d066a42a47a0edb22216", "start_char": 0, "end_char": 1531, "text_sha256": "1723620d459b4f48ad397d59284df3f0619894ba82c6d066a42a47a0edb22216"} [gb-p8621444] The novel anticonvulsant drug, gabapentin (Neurontin), binds to the alpha2delta subunit of a calcium channel. (1996). https://pubmed.ncbi.nlm.nih.gov/8621444/ DOI: 10.1074/jbc.271.10.5768
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.