Component
Smoothened / SMO
Smoothened / SMO. Species, exposure and limitations are retained in each linked claim.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
Where it participates (unsigned role)
Mebendazole potently inhibited hedgehog signalling and slowed the growth of hedgehog-driven human medulloblastoma cells at clinically attainable concentrations, suppressed formation of the primary cilium, a microtubule-based organelle that functions as a signalling hub for pathway activation, and its inhibition was unaffected by mutants in the gene encoding human Smoothened that are selectively propagated in clones surviving vismodegib, with the combination of vismodegib and mebendazole giving additive inhibition.
Experimental context and source evidence
- evidence_span
- {"source_cache": "artifacts/mebendazole-research/25376612.abstract.txt", "locator": "Indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "fa6b6f06dfda62c5f1cc59df64f813c5d112f8d6a3bf969c6fa4432073a87ec0", "start_char": 0, "end_char": 1290, "text_sha256": "fa6b6f06dfda62c5f1cc59df64f813c5d112f8d6a3bf969c6fa4432073a87ec0"}
- experimental_model
- Hedgehog reporter and medulloblastoma cell growth assays with Smoothened mutant lines
- exposure
- Mebendazole at clinically attainable concentrations, alone and with vismodegib
- limitations
- Proposes a second mechanism which is still microtubule-based, through the primary cilium. Cell assays; the claim that the concentrations are clinically attainable is the authors’.
- nutrient_topic
- Mebendazole research collection; topical membership is not evidence of a direct clinical effect, and mebendazole is recorded separately from albendazole, from the benzimidazole class and from its own crystal forms. · Mebendazole
- organism
- Human cells
- plain_language
- It blocks this growth pathway by removing the little antenna the pathway needs, so mutations that defeat the standard drug do not defeat it.
- primary_references
- [mbz-p25376612] Repurposing the antihelmintic mebendazole as a hedgehog inhibitor. (2015). https://pubmed.ncbi.nlm.nih.gov/25376612/ DOI: 10.1158/1535-7163.mct-14-0755-t
- tissue_or_cell_type
- Medulloblastoma
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Hedgehog reporter and medulloblastoma cell growth assays with Smoothened mutant lines · source_derived_draft · unverified_draft
### mbz-hedgehog-through-the-cilium Mebendazole potently inhibited hedgehog signalling and slowed the growth of hedgehog-driven human medulloblastoma cells at clinically attainable concentrations, suppressed formation of the primary cilium, a microtubule-based organelle that functions as a signalling hub for pathway activation, and its inhibition was unaffected by mutants in the gene encoding human Smoothened that are selectively propagated in clones surviving vismodegib, with the combination of vismodegib and mebendazole giving additive inhibition. Condition category: normal nutrient_topic: Mebendazole research collection; topical membership is not evidence of a direct clinical effect, and mebendazole is recorded separately from albendazole, from the benzimidazole class and from its own crystal forms. plain_language: It blocks this growth pathway by removing the little antenna the pathway needs, so mutations that defeat the standard drug do not defeat it. organism: Human cells tissue_or_cell_type: Medulloblastoma experimental_model: Hedgehog reporter and medulloblastoma cell growth assays with Smoothened mutant lines limitations: Proposes a second mechanism which is still microtubule-based, through the primary cilium. Cell assays; the claim that the concentrations are clinically attainable is the authors’. exposure: Mebendazole at clinically attainable concentrations, alone and with vismodegib evidence_span: {"source_cache": "artifacts/mebendazole-research/25376612.abstract.txt", "locator": "Indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "fa6b6f06dfda62c5f1cc59df64f813c5d112f8d6a3bf969c6fa4432073a87ec0", "start_char": 0, "end_char": 1290, "text_sha256": "fa6b6f06dfda62c5f1cc59df64f813c5d112f8d6a3bf969c6fa4432073a87ec0"} [mbz-p25376612] Repurposing the antihelmintic mebendazole as a hedgehog inhibitor. (2015). https://pubmed.ncbi.nlm.nih.gov/25376612/ DOI: 10.1158/1535-7163.mct-14-0755-t
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.