Component
Rat branched-chain ketoacid dehydrogenase kinase
Rattus norvegicus regulatory kinase used as a recombinant protein in mixed-species E1b phosphorylation experiments.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
Where it participates (unsigned role)
ThDP reduced kinase-mediated phosphorylation of wild-type human E1b; loop-network mutations abolished this protection in the reconstituted assay.
Experimental context and source evidence
- evidence
- [{"paper_key": "li-2004-bckdh", "source_bundle": "artifacts/thiamine_metabolism_sources.json", "passage_ids": ["abstract"], "locator": "Primary publication abstract", "preservation": "Exact text retained in the source bundle; full source document retained when openly retrievable."}]
- experimental_model
- Human E1b/E2b with recombinant rat BCKD kinase.
- limitations
- Mixed-species assay; kinase accessibility, not clinical thiamine responsiveness.
- nutrient
- Thiamine (vitamin B1) · Thiamine (vitamin B1)
- nutrient_topic
- Thiamine research collection; topical membership is not evidence of a direct dietary effect. · Thiamine (vitamin B1)
- organism
- Homo sapiens E1/E2; Rattus norvegicus kinase
- plain_language
- Cofactor occupancy also affected access to a regulatory site. The result depends on an intact loop and does not show that every genetic BCKDH defect responds to B1.
- primary_references
- [li-2004-bckdh] Cross-talk between thiamin diphosphate binding and phosphorylation loop conformation in human branched-chain alpha-keto acid decarboxylase/dehydrogenase (2004). https://pubmed.ncbi.nlm.nih.gov/15166214/ DOI: 10.1074/jbc.m403611200
- tissue_or_cell_type
- Purified mixed-species assay
Thiamine: mechanisms, deficiency and nutrient interactions (2026-09-17) · lines 801–812
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human E1b/E2b with recombinant rat BCKD kinase. · source_derived_draft · unverified_draft
### b1-bckdh-cofactor-limits-phosphorylation ThDP reduced kinase-mediated phosphorylation of wild-type human E1b; loop-network mutations abolished this protection in the reconstituted assay. Condition category: normal nutrient_topic: Thiamine research collection; topical membership is not evidence of a direct dietary effect. plain_language: Cofactor occupancy also affected access to a regulatory site. The result depends on an intact loop and does not show that every genetic BCKDH defect responds to B1. organism: Homo sapiens E1/E2; Rattus norvegicus kinase tissue_or_cell_type: Purified mixed-species assay experimental_model: Human E1b/E2b with recombinant rat BCKD kinase. limitations: Mixed-species assay; kinase accessibility, not clinical thiamine responsiveness. evidence: [{"paper_key": "li-2004-bckdh", "source_bundle": "artifacts/thiamine_metabolism_sources.json", "passage_ids": ["abstract"], "locator": "Primary publication abstract", "preservation": "Exact text retained in the source bundle; full source document retained when openly retrievable."}] nutrient: Thiamine (vitamin B1) [li-2004-bckdh] Cross-talk between thiamin diphosphate binding and phosphorylation loop conformation in human branched-chain alpha-keto acid decarboxylase/dehydrogenase (2004). https://pubmed.ncbi.nlm.nih.gov/15166214/ DOI: 10.1074/jbc.m403611200
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.