Component
Mouse BCO1 E405A
Independent engineered alanine-substitution state of mouse BCO1.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
Where it participates (unsigned role)
Substitution of conserved histidines or Glu405 in mouse BCO1 abolished cleavage and reduced protein-bound iron.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- cross_nutrient
- Links enzyme-bound iron to vitamin A precursor processing.
- evidence_location
- Abstract
- experimental_model
- Site-directed mutant mouse BCO1; cleavage assays and protein-bound iron spectroscopy.
- exposure
- Individual alanine substitutions at conserved catalytic-site residues.
- limitations
- Coordination inferred from mutagenesis and iron measurements, not a dietary iron trial.
- nutrient_topic
- Vitamin A research collection; topical membership is not evidence of a direct dietary effect. · Vitamin A
- organism
- Mus musculus recombinant protein
- outcome
- Substitution of conserved histidines or Glu405 in mouse BCO1 abolished cleavage and reduced protein-bound iron.
- plain_language
- An intact iron-binding environment is necessary for this enzyme to work.
- primary_references
- [va-poliakov-2005] Key role of conserved histidines in recombinant mouse beta-carotene 15,15'-monooxygenase-1 activity (2005). https://pubmed.ncbi.nlm.nih.gov/15951442/ DOI: 10.1074/jbc.M500409200
- tissue_or_cell_type
- Cell-free protein
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Vitamin A: forms, mechanisms, deficiency and excess (2026-09-17) · lines 217–230
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Site-directed mutant mouse BCO1; cleavage assays and protein-bound iron spectroscopy. · source_derived_draft · unverified_draft
### va-bco1-iron-binding-machinery Substitution of conserved histidines or Glu405 in mouse BCO1 abolished cleavage and reduced protein-bound iron. Condition category: machinery_impairment nutrient_topic: Vitamin A research collection; topical membership is not evidence of a direct dietary effect. plain_language: An intact iron-binding environment is necessary for this enzyme to work. organism: Mus musculus recombinant protein tissue_or_cell_type: Cell-free protein experimental_model: Site-directed mutant mouse BCO1; cleavage assays and protein-bound iron spectroscopy. limitations: Coordination inferred from mutagenesis and iron measurements, not a dietary iron trial. exposure: Individual alanine substitutions at conserved catalytic-site residues. outcome: Substitution of conserved histidines or Glu405 in mouse BCO1 abolished cleavage and reduced protein-bound iron. evidence_location: Abstract cross_nutrient: Links enzyme-bound iron to vitamin A precursor processing. [va-poliakov-2005] Key role of conserved histidines in recombinant mouse beta-carotene 15,15'-monooxygenase-1 activity (2005). https://pubmed.ncbi.nlm.nih.gov/15951442/ DOI: 10.1074/jbc.M500409200
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.