Component
Synthesis of 12-oxo-phytodienoic acid and jasmonates in plants
Synthesis of 12-oxo-phytodienoic acid and jasmonates in plants. Species, exposure and limitations are retained in each linked claim.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
Where it participates (unsigned role)
Aspirin causes time-dependent inhibition and acetylation of plant allene oxide synthase leading to irreversible inactivation of this cytochrome P450, acetylating three serine residues near the C-terminal region that are highly conserved among allene oxide synthases but not among classical P450s; unlike animal cyclooxygenase, where acetylation of a single serine within the substrate channel causes inactivation, these three serines are not thought to line the putative substrate channel, so inhibition may be by a different mechanism, and aspirin could inhibit other P450s with similar motifs.
Experimental context and source evidence
- evidence_span
- {"source_cache": "artifacts/aspirin-research/9660772.abstract.txt", "locator": "Indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "49e5d050a7b1505f537ae535df4e7a51563a72d528973779baf53c963b50c259", "start_char": 0, "end_char": 1662, "text_sha256": "49e5d050a7b1505f537ae535df4e7a51563a72d528973779baf53c963b50c259"}
- experimental_model
- Inhibition and acetylation of plant allene oxide synthase, a cytochrome P450
- exposure
- Aspirin and salicylic acid applied to the plant oxylipin pathway
- limitations
- Recorded because it shows the acetyl group is not specific to cyclooxygenase. The three acetylated serines here are not thought to line the substrate channel, so the mechanism may differ from the one at cyclooxygenase.
- nutrient_topic
- Aspirin research collection; topical membership is not evidence of a direct clinical effect, and aspirin is recorded separately from salicylate, the metabolite it becomes. · Aspirin / acetylsalicylic acid
- organism
- Plant
- plain_language
- Aspirin hands its acetyl group to other enzymes too, including one in plants, and not always at the substrate channel.
- primary_references
- [asa-p9660772] Aspirin inhibition and acetylation of the plant cytochrome P450, allene oxide synthase, resembles that of animal prostaglandin endoperoxide H synthase. (1998). https://pubmed.ncbi.nlm.nih.gov/9660772/ DOI: 10.1074/jbc.273.29.18139
- tissue_or_cell_type
- Allene oxide synthase
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Inhibition and acetylation of plant allene oxide synthase, a cytochrome P450 · source_derived_draft · unverified_draft
### asa-acetylates-other-enzymes Aspirin causes time-dependent inhibition and acetylation of plant allene oxide synthase leading to irreversible inactivation of this cytochrome P450, acetylating three serine residues near the C-terminal region that are highly conserved among allene oxide synthases but not among classical P450s; unlike animal cyclooxygenase, where acetylation of a single serine within the substrate channel causes inactivation, these three serines are not thought to line the putative substrate channel, so inhibition may be by a different mechanism, and aspirin could inhibit other P450s with similar motifs. Condition category: normal nutrient_topic: Aspirin research collection; topical membership is not evidence of a direct clinical effect, and aspirin is recorded separately from salicylate, the metabolite it becomes. plain_language: Aspirin hands its acetyl group to other enzymes too, including one in plants, and not always at the substrate channel. organism: Plant tissue_or_cell_type: Allene oxide synthase experimental_model: Inhibition and acetylation of plant allene oxide synthase, a cytochrome P450 limitations: Recorded because it shows the acetyl group is not specific to cyclooxygenase. The three acetylated serines here are not thought to line the substrate channel, so the mechanism may differ from the one at cyclooxygenase. exposure: Aspirin and salicylic acid applied to the plant oxylipin pathway evidence_span: {"source_cache": "artifacts/aspirin-research/9660772.abstract.txt", "locator": "Indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "49e5d050a7b1505f537ae535df4e7a51563a72d528973779baf53c963b50c259", "start_char": 0, "end_char": 1662, "text_sha256": "49e5d050a7b1505f537ae535df4e7a51563a72d528973779baf53c963b50c259"} [asa-p9660772] Aspirin inhibition and acetylation of the plant cytochrome P450, allene oxide synthase, resembles that of animal prostaglandin endoperoxide H synthase. (1998). https://pubmed.ncbi.nlm.nih.gov/9660772/ DOI: 10.1074/jbc.273.29.18139
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.