Component
2- and 3-hydroxyibuprofen
2- and 3-hydroxyibuprofen. Species, exposure and limitations are retained in each linked claim.
1 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
Where it participates (unsigned role)
Complementary-DNA-expressed CYP2C9 favoured formation of S-2- and S-3-hydroxyibuprofen while CYP2C8 favoured R-2-hydroxyibuprofen formation, high-affinity Km values for S-ibuprofen hydroxylation were 38 and 21 micromolar against 47 and 29 micromolar for R-ibuprofen, sulfaphenazole competitively inhibited all four hydroxylations with submicromolar Ki values, and the regio- and stereoselectivities observed in vitro were consistent with those noted in vivo.
Experimental context and source evidence
- evidence_span
- {"source_cache": "artifacts/ibuprofen-research/9296349.abstract.txt", "locator": "Indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "19179a5f8220c1d4037113812f8968e2730ecf07c6ad94997d2661d7c6c75afb", "start_char": 0, "end_char": 2012, "text_sha256": "19179a5f8220c1d4037113812f8968e2730ecf07c6ad94997d2661d7c6c75afb"}
- experimental_model
- Human liver microsomes and complementary-DNA-expressed cytochromes with a bank of fourteen microsomal preparations
- exposure
- Both enantiomers of ibuprofen assayed for 2- and 3-hydroxylation, with sulfaphenazole, retinol and arachidonic acid as inhibitors
- limitations
- Assigns each enantiomer to a different cytochrome by direct comparison in the same system. In vitro microsomes, though the authors note the selectivities matched those seen in vivo.
- nutrient_topic
- Ibuprofen research collection; topical membership is not evidence of a direct clinical effect, and the racemate is recorded separately from each of its two enantiomers. · Ibuprofen
- organism
- Human
- plain_language
- The body uses two different clearance enzymes for the two hands of the same molecule.
- primary_references
- [ibu-p9296349] Regioselective and stereoselective metabolism of ibuprofen by human cytochrome P450 2C. (1997). https://pubmed.ncbi.nlm.nih.gov/9296349/ DOI: 10.1016/s0006-2952(97)00143-3
- tissue_or_cell_type
- Liver microsomes
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human liver microsomes and complementary-DNA-expressed cytochromes with a bank of fourteen microsomal preparations · source_derived_draft · unverified_draft
### ibu-different-cytochromes Complementary-DNA-expressed CYP2C9 favoured formation of S-2- and S-3-hydroxyibuprofen while CYP2C8 favoured R-2-hydroxyibuprofen formation, high-affinity Km values for S-ibuprofen hydroxylation were 38 and 21 micromolar against 47 and 29 micromolar for R-ibuprofen, sulfaphenazole competitively inhibited all four hydroxylations with submicromolar Ki values, and the regio- and stereoselectivities observed in vitro were consistent with those noted in vivo. Condition category: normal nutrient_topic: Ibuprofen research collection; topical membership is not evidence of a direct clinical effect, and the racemate is recorded separately from each of its two enantiomers. plain_language: The body uses two different clearance enzymes for the two hands of the same molecule. organism: Human tissue_or_cell_type: Liver microsomes experimental_model: Human liver microsomes and complementary-DNA-expressed cytochromes with a bank of fourteen microsomal preparations limitations: Assigns each enantiomer to a different cytochrome by direct comparison in the same system. In vitro microsomes, though the authors note the selectivities matched those seen in vivo. exposure: Both enantiomers of ibuprofen assayed for 2- and 3-hydroxylation, with sulfaphenazole, retinol and arachidonic acid as inhibitors evidence_span: {"source_cache": "artifacts/ibuprofen-research/9296349.abstract.txt", "locator": "Indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "19179a5f8220c1d4037113812f8968e2730ecf07c6ad94997d2661d7c6c75afb", "start_char": 0, "end_char": 2012, "text_sha256": "19179a5f8220c1d4037113812f8968e2730ecf07c6ad94997d2661d7c6c75afb"} [ibu-p9296349] Regioselective and stereoselective metabolism of ibuprofen by human cytochrome P450 2C. (1997). https://pubmed.ncbi.nlm.nih.gov/9296349/ DOI: 10.1016/s0006-2952(97)00143-3
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.