{"id":"fff2a32e-38f1-5312-ab0c-65bf1e71c26a","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-hl60-nqo1","predicate":"increases","statement":"NQO1 protein and enzyme activity increased after lipoic-acid treatment in HL-60 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"71de82b9-a39f-5f90-b4af-f84aa0835692","mechanism_event_label":"The response included more activity of a specific quinone-processing enzyme.","subject":{"id":"ab091982-3acb-5a87-80fb-85dfe292c1e0","slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"},"object":{"id":"01f73291-ab25-59e2-a5e9-71d15a6c2a0a","slug":"nqo1","display_name":"Human NAD(P)H quinone dehydrogenase 1 / NQO1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"71de82b9-a39f-5f90-b4af-f84aa0835692","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-hl60-nqo1-event","event_type":"biochemical_relationship","label":"The response included more activity of a specific quinone-processing enzyme.","description":"NQO1 protein and enzyme activity increased after lipoic-acid treatment in HL-60 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dc472dd3-d383-567a-ab37-a9ec4e112df4","slug":"nfe2l2","display_name":"Human Nrf2 / NFE2L2","entity_type_key":"protein"},"role":"associated_regulator","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ab091982-3acb-5a87-80fb-85dfe292c1e0","slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"01f73291-ab25-59e2-a5e9-71d15a6c2a0a","slug":"nqo1","display_name":"Human NAD(P)H quinone dehydrogenase 1 / NQO1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/ala-research/18813798.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"bcc2d2fa7c8f550c53af52dfcd88a1e84bee38f98b85cb72e723eba495b01814\", \"start_char\": 0, \"end_char\": 1245, \"text_sha256\": \"bcc2d2fa7c8f550c53af52dfcd88a1e84bee38f98b85cb72e723eba495b01814\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human HL-60 leukemia cell biochemical study","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Alpha-lipoic-acid exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Association of Nrf2/KEAP1 changes with NQO1 induction does not by itself establish genetic dependency or clinical cancer benefit.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human cell line","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The response included more activity of a specific quinone-processing enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ala-p18813798] Control of cellular redox status and upregulation of quinone reductase NQO1 via Nrf2 activation by alpha-lipoic acid in human leukemia HL-60 cells. (2008). https://pubmed.ncbi.nlm.nih.gov/18813798/ DOI: 10.3892/ijo_00000071","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HL-60 cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"14570123-820e-5b84-be0c-f95da290bfce","evidence_kind":"source_excerpt","locator":"Lines 884-895","start_line":884,"end_line":895,"excerpt":"### ala-hl60-nqo1\nNQO1 protein and enzyme activity increased after lipoic-acid treatment in HL-60 cells.\nCondition category: normal\nnutrient_topic: Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The response included more activity of a specific quinone-processing enzyme.\norganism: Human cell line\ntissue_or_cell_type: HL-60 cells\nexperimental_model: Human HL-60 leukemia cell biochemical study\nlimitations: Association of Nrf2/KEAP1 changes with NQO1 induction does not by itself establish genetic dependency or clinical cancer benefit.\nexposure: Alpha-lipoic-acid exposure\nevidence_span: {\"source_cache\": \"artifacts/ala-research/18813798.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"bcc2d2fa7c8f550c53af52dfcd88a1e84bee38f98b85cb72e723eba495b01814\", \"start_char\": 0, \"end_char\": 1245, \"text_sha256\": \"bcc2d2fa7c8f550c53af52dfcd88a1e84bee38f98b85cb72e723eba495b01814\"}\n[ala-p18813798] Control of cellular redox status and upregulation of quinone reductase NQO1 via Nrf2 activation by alpha-lipoic acid in human leukemia HL-60 cells. 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