{"id":"fff14f09-1925-5fcb-a93b-3202916e677f","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-sqle-thiol-reversal","predicate":"changes_tested","statement":"Thiol-containing reagents, including glutathione, prevented or reversed inhibition in the garlic-compound SQLE assay series.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"42937040-3dc4-54d0-a577-15c20aa26ba2","mechanism_event_label":"The enzyme result connects to thiol chemistry.","subject":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"object":{"id":"0e02c502-ace0-5f2d-bf25-5c5351035cc8","slug":"sqle-garlic-inhibition-reversal","display_name":"Thiol reversal of garlic-compound SQLE inhibition","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"42937040-3dc4-54d0-a577-15c20aa26ba2","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-sqle-thiol-reversal-event","event_type":"observed_relationship","label":"The enzyme result connects to thiol chemistry.","description":"Thiol-containing reagents, including glutathione, prevented or reversed inhibition in the garlic-compound SQLE assay series.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0e02c502-ace0-5f2d-bf25-5c5351035cc8","slug":"sqle-garlic-inhibition-reversal","display_name":"Thiol reversal of garlic-compound SQLE inhibition","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"923a6f4a-e004-5fa5-a434-5e7f78c543c2","slug":"sqle","display_name":"Human squalene monooxygenase / SQLE","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"acting_entity","value_text":"glutathione","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"SAC IC50 110 micromolar","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Time-dependent inhibition; duration not retrieved","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_comparison","value_text":"SAC and other compounds tested individually; thiol reversal experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified recombinant squalene monooxygenase assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived research curation; not independent primary verification","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The abstract groups several inhibitors. SAC-specific reversal magnitude and a covalent site are not separately established; dithiols were more effective than monothiols.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"S-allylcysteine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human recombinant protein","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The enzyme result connects to thiol chemistry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[11385050] Garlic and garlic-derived compounds inhibit human squalene monooxygenase. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11385050/ · DOI 10.1093/jn/131.6.1662","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Cell-free enzyme assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified recombinant squalene monooxygenase assay","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4dfcbbeb-241f-5885-87b4-e83fe77c85da","evidence_kind":"source_excerpt","locator":"Lines 230-237","start_line":230,"end_line":237,"excerpt":"## s-allylcysteine-sqle-thiol-reversal\nThe enzyme result connects to thiol chemistry.\nThiol-containing reagents, including glutathione, prevented or reversed inhibition in the garlic-compound SQLE assay series.\nModel: Purified recombinant squalene monooxygenase assay\nLimitations: The abstract groups several inhibitors. SAC-specific reversal magnitude and a covalent site are not separately established; dithiols were more effective than monothiols.\nEvidence access: Primary abstract\n[11385050] Garlic and garlic-derived compounds inhibit human squalene monooxygenase. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11385050/ · DOI 10.1093/jn/131.6.1662\nStructured context: {\"organism\": \"Human recombinant protein\", \"tissue_or_cell_type\": \"Purified recombinant squalene monooxygenase assay\", \"dose\": \"SAC IC50 110 micromolar\", \"duration\": \"Time-dependent inhibition; duration not retrieved\", \"route\": \"Cell-free enzyme assay\", \"experimental_comparison\": \"SAC and other compounds tested individually; thiol reversal experiments\", \"acting_entity\": \"glutathione\", \"interpretation_status\": \"Source-derived research curation; not independent primary verification\"}","model_system":"Purified recombinant squalene monooxygenase assay","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3b0d58f9-8a69-579d-9920-518399a9a8bb","stable_key":"import-d96b230c-481b-535b-930c-04eb87ec7f02","title":"S-allylcysteine: sulfur signaling, redox responses and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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