{"id":"ff9caafa-af32-544a-a3e0-2773ba449903","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-marc1-nreduction","predicate":"reduces","statement":"Recombinant human mARC1 reduced N-hydroxylated compounds, including N-hydroxycytosine.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"aaf10ab0-e2a9-5062-b890-e416a6e90064","mechanism_event_label":"mARC1 removes oxygen from selected nitrogen-containing molecules.","subject":{"id":"cd629c45-47a6-555e-9ad9-9abb93a04e7f","slug":"mtarc1","display_name":"Human mitochondrial amidoxime-reducing component 1 / MTARC1","entity_type_key":"protein"},"object":{"id":"7c62255c-1730-54ea-a259-ef2f8d886f8c","slug":"n-hydroxycytosine","display_name":"N-Hydroxycytosine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"aaf10ab0-e2a9-5062-b890-e416a6e90064","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-marc1-nreduction-event","event_type":"biochemical_relationship","label":"mARC1 removes oxygen from selected nitrogen-containing molecules.","description":"Recombinant human mARC1 reduced N-hydroxylated compounds, including N-hydroxycytosine.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ac4bcc03-66fb-52c1-b191-9ecb2124659f","slug":"molybdenum-cofactor","display_name":"Molybdenum cofactor / Moco","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cd629c45-47a6-555e-9ad9-9abb93a04e7f","slug":"mtarc1","display_name":"Human mitochondrial amidoxime-reducing component 1 / MTARC1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7c62255c-1730-54ea-a259-ef2f8d886f8c","slug":"n-hydroxycytosine","display_name":"N-Hydroxycytosine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/molybdenum-research/20861021.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0f430936dcc4701e5d57040edcab4b0418e3c49c59620e0f7d5f54aff41e7877\", \"start_char\": 0, \"end_char\": 1557, \"text_sha256\": \"0f430936dcc4701e5d57040edcab4b0418e3c49c59620e0f7d5f54aff41e7877\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human mARC1/mARC2 biochemical and spectroscopic reconstitution","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"N-hydroxylated substrates; cofactor reconstitution","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The 2010 paper could not identify the Mo-ligating cysteine; later structural/mutagenesis work resolves that point. Its earlier inference is not imported as current fact.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Molybdenum research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"molybdenum","display_name":"Molybdenum","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human proteins expressed in Escherichia coli","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"mARC1 removes oxygen from selected nitrogen-containing molecules.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mo-p20861021] Biochemical and spectroscopic characterization of the human mitochondrial amidoxime reducing components hmARC-1 and hmARC-2 suggests the existence of a new molybdenum enzyme family in eukaryotes. (2010). https://pubmed.ncbi.nlm.nih.gov/20861021/ DOI: 10.1074/jbc.m110.169532","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified enzyme system","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"589e6b53-9dbd-58e5-b8cf-63ad6427b067","evidence_kind":"source_excerpt","locator":"Lines 937-948","start_line":937,"end_line":948,"excerpt":"### mo-marc1-nreduction\nRecombinant human mARC1 reduced N-hydroxylated compounds, including N-hydroxycytosine.\nCondition category: normal\nnutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: mARC1 removes oxygen from selected nitrogen-containing molecules.\norganism: Human proteins expressed in Escherichia coli\ntissue_or_cell_type: Purified enzyme system\nexperimental_model: Recombinant human mARC1/mARC2 biochemical and spectroscopic reconstitution\nlimitations: The 2010 paper could not identify the Mo-ligating cysteine; later structural/mutagenesis work resolves that point. Its earlier inference is not imported as current fact.\nexposure: N-hydroxylated substrates; cofactor reconstitution\nevidence_span: {\"source_cache\": \"artifacts/molybdenum-research/20861021.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0f430936dcc4701e5d57040edcab4b0418e3c49c59620e0f7d5f54aff41e7877\", \"start_char\": 0, \"end_char\": 1557, \"text_sha256\": \"0f430936dcc4701e5d57040edcab4b0418e3c49c59620e0f7d5f54aff41e7877\"}\n[mo-p20861021] Biochemical and spectroscopic characterization of the human mitochondrial amidoxime reducing components hmARC-1 and hmARC-2 suggests the existence of a new molybdenum enzyme family in eukaryotes. (2010). https://pubmed.ncbi.nlm.nih.gov/20861021/ DOI: 10.1074/jbc.m110.169532","model_system":"Recombinant human mARC1/mARC2 biochemical and spectroscopic reconstitution","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mo-p20861021] Biochemical and spectroscopic characterization of the human mitochondrial amidoxime reducing components hmARC-1 and hmARC-2 suggests the existence of a new molybdenum enzyme family in eukaryotes. (2010). https://pubmed.ncbi.nlm.nih.gov/20861021/ DOI: 10.1074/jbc.m110.169532","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1aa6da60-8284-5252-8dd9-ac2250d5ced5","stable_key":"import-dc8975b1-95ff-5d9b-be17-a1c04610cca7","title":"Molybdenum: cofactor assembly, sulfur metabolism and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"143147eb9ac7fe750bdd33bb50d7773bc0cd49cc94faf219aebce3c3d760dd8f","revision_id":"90c2239d-290b-5e16-815a-f94d30569ebd","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}