{"id":"ff925e86-83d3-5a01-8b75-0868bd651bf3","stable_key":"33bef151-bdc7-5892-aff6-0345ab149739:aprn-encodes-the-subtilisin-nat-preproenzyme","predicate":"encodes","statement":"The aprN gene of Bacillus subtilis (natto) encodes the subtilisin NAT preproenzyme.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"bcb41847-5866-5175-adf5-13fafcc79389","mechanism_event_label":"The aprN gene of Bacillus subtilis (natto) encodes the subtilisin NAT preproenzyme.","subject":{"id":"6ff23556-826d-5da3-83e8-92cc6d071148","slug":"aprn","display_name":"Bacillus subtilis natto aprN gene","entity_type_key":"gene"},"object":{"id":"c2897a41-6467-5457-b761-77d4d199524e","slug":"subtilisin-nat-precursor","display_name":"Subtilisin NAT preproenzyme","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"bcb41847-5866-5175-adf5-13fafcc79389","stable_key":"33bef151-bdc7-5892-aff6-0345ab149739:aprn-encodes-the-subtilisin-nat-preproenzyme-event","event_type":"biochemical_relationship","label":"The aprN gene of Bacillus subtilis (natto) encodes the subtilisin NAT preproenzyme.","description":"Bacterial gene expression","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6ff23556-826d-5da3-83e8-92cc6d071148","slug":"aprn","display_name":"Bacillus subtilis natto aprN gene","entity_type_key":"gene"},"role":"acting component","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c2897a41-6467-5457-b761-77d4d199524e","slug":"subtilisin-nat-precursor","display_name":"Subtilisin NAT preproenzyme","entity_type_key":"protein"},"role":"component the finding is about","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Bacillus subtilis var. natto","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Not applicable","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"PubMed carries no abstract for this record, so only the nucleotide-sequence result named in the title is taken from it. The commonly quoted 29-residue signal peptide and 77-residue propeptide lengths are not sourced by anything read during this curation and are recorded as an open question instead.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Bacillus subtilis var. natto","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The aprN gene of Bacillus subtilis (natto) encodes the subtilisin NAT preproenzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Nucleotide sequence of the subtilisin NAT gene, aprN, of Bacillus subtilis (natto). (1992) https://pubmed.ncbi.nlm.nih.gov/1369081/ DOI: 10.1271/bbb.56.1869","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Bacterial gene expression","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6ba8929c-8eef-5948-9667-6b6992125a5d","evidence_kind":"source_excerpt","locator":"Lines 37-37","start_line":37,"end_line":37,"excerpt":"The aprN gene of Bacillus subtilis (natto) encodes the subtilisin NAT preproenzyme.","model_system":"Bacillus subtilis var. natto","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"41cb127c-1c23-53aa-b7d0-5ec7f6fa3c99","stable_key":"import-33bef151-bdc7-5892-aff6-0345ab149739","title":"Nattokinase: what the purified enzyme cleaves, what survives being eaten, and the gap between the two (2026-09-23)","document_type":"imported_text","citation_label":"Original AI-assisted curation built from a supplied entity-first document of 105 entities and 129 claims. Every reference in that document was resolved against live PubMed with its abstract read and its DOI cross-checked on 2026-09-23, and the EFSA novel-food opinion was retrieved and read in full. That check corrected two PMIDs that pointed at unrelated papers, two DOIs, and two papers recorded as carrying no erratum that do carry one; it also reversed three findings the supplied document had stated backwards. Two papers carry a published correction, recorded as such and not as a retraction. Three sources are not indexed in PubMed and are cited by what they have. Laboratory lineages are recorded, so the four papers from one group, the three from another and the two readings of a single applicant dossier cannot be counted as separate lines of support. Study-specific doses, units, populations and limitations retained; activity units are never converted between systems. Not publisher full text.","file_path":"","sha256":"34fc46e0e849d06bfa13cb099a79b50679dd6fd0de8a5a0575326afdde3259c1","revision_id":"efd063e6-e982-5f6e-a4b8-6f2ffe4185ce","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}