{"id":"ff8993ee-cc97-506a-955d-9eb8839797a4","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-rat-titin","predicate":"increases_phosphorylation","statement":"Spermidine increased titin phosphorylation in high-salt-fed Dahl salt-sensitive rats.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"cec485f9-1078-50ac-9fe4-64014ffbf7c9","mechanism_event_label":"A structural heart protein changed alongside cardiac function.","subject":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"object":{"id":"fa6853fb-c3f6-5424-b7aa-f7472d65b21c","slug":"rat-ttn","display_name":"Rat titin / Ttn","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"cec485f9-1078-50ac-9fe4-64014ffbf7c9","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-rat-titin-event","event_type":"observed_relationship","label":"A structural heart protein changed alongside cardiac function.","description":"Spermidine increased titin phosphorylation in high-salt-fed Dahl salt-sensitive rats.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fa6853fb-c3f6-5424-b7aa-f7472d65b21c","slug":"rat-ttn","display_name":"Rat titin / Ttn","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Hypertension/heart-failure rat model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not a demonstrated direct kinase target; blood-pressure reduction may contribute.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A structural heart protein changed alongside cardiac function.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cardioprotection and lifespan extension by the natural polyamine spermidine. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27841876/ · DOI 10.1038/nm.4222","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"91f8f79c-5cbf-580a-86ba-337ec36b05d5","evidence_kind":"source_excerpt","locator":"Lines 342-348","start_line":342,"end_line":348,"excerpt":"## spermidine-rat-titin\nA structural heart protein changed alongside cardiac function.\nSpermidine increased titin phosphorylation in high-salt-fed Dahl salt-sensitive rats.\nModel: Hypertension/heart-failure rat model.\nLimitations: Not a demonstrated direct kinase target; blood-pressure reduction may contribute.\nEvidence access: Primary abstract\nCardioprotection and lifespan extension by the natural polyamine spermidine. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27841876/ · DOI 10.1038/nm.4222","model_system":"Hypertension/heart-failure rat model.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8eec8784bc2bd0384cfd53c6603c4ced8e53c227d9ab29ed85594c43e34c68ef","revision_id":"7c421a18-4526-59f6-b84e-4be533bc35ac","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}