{"id":"ff1f51a9-1536-5ee7-ac6b-cc0f4f860f10","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-parent-cyp2c19","predicate":"has_limited_effect","statement":"At 20 micromolar, the parent did not significantly inhibit mephenytoin hydroxylation in the human CYP2C19 assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"fefca5d8-72d0-5bfa-9749-5a3405995204","mechanism_event_label":"A transporter interaction does not imply strong CYP inhibition.","subject":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"object":{"id":"cc080b54-fd5d-5cc0-930e-2cb86c116c68","slug":"cyp2c19","display_name":"Human cytochrome P450 2C19","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"fefca5d8-72d0-5bfa-9749-5a3405995204","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-parent-cyp2c19-event","event_type":"observed_relationship","label":"A transporter interaction does not imply strong CYP inhibition.","description":"At 20 micromolar, the parent did not significantly inhibit mephenytoin hydroxylation in the human CYP2C19 assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cc080b54-fd5d-5cc0-930e-2cb86c116c68","slug":"cyp2c19","display_name":"Human cytochrome P450 2C19","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human enzymes; 5 micromolar substrate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Restricted substrate, concentration and preparation; earlier CYP2C9 assays gave different potency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Myricetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A transporter interaction does not imply strong CYP inhibition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Interaction of myricetin, ampelopsin (dihydromyricetin), and their sulfate metabolites with serum albumin, cytochrome P450 (CYP2C9, 2C19, and 3A4) enzymes, and organic anion-transporting polypeptides (OATP1B1 and OATP2B1). · 2024 · https://pubmed.ncbi.nlm.nih.gov/39344282/ · DOI 10.1002/prp2.70021","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"df203b74-bf03-5091-8e8e-6344e48c4209","evidence_kind":"source_excerpt","locator":"Lines 140-146","start_line":140,"end_line":146,"excerpt":"## myricetin-parent-cyp2c19\nA transporter interaction does not imply strong CYP inhibition.\nAt 20 micromolar, the parent did not significantly inhibit mephenytoin hydroxylation in the human CYP2C19 assay.\nModel: Recombinant human enzymes; 5 micromolar substrate.\nLimitations: Restricted substrate, concentration and preparation; earlier CYP2C9 assays gave different potency.\nEvidence access: Primary full text\nInteraction of myricetin, ampelopsin (dihydromyricetin), and their sulfate metabolites with serum albumin, cytochrome P450 (CYP2C9, 2C19, and 3A4) enzymes, and organic anion-transporting polypeptides (OATP1B1 and OATP2B1). · 2024 · https://pubmed.ncbi.nlm.nih.gov/39344282/ · DOI 10.1002/prp2.70021","model_system":"Recombinant human enzymes; 5 micromolar substrate.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"58045cd9-3b27-5469-bb4b-e1c6a9bdca34","stable_key":"import-8182578e-8d21-598e-a3cb-e62d480d2251","title":"Myricetin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"0abf512275cf3aae9e4ec8544f1cde97e0155d0cfb5be12a193cdd5a898386a7","revision_id":"422e342f-a55c-5e73-ba98-43df560f49b9","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}