{"id":"ff05c9dd-b071-55ef-9c46-47e6c76dbeea","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-taucl-no","predicate":"reduces","statement":"Adding 0.5 mM taurine chloramine to activated RAW264.7 macrophages reduced measured nitrite output by 47%; cytosolic nitric-oxide-synthase activity was also inhibited by pretreatment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"021be326-ace8-57c5-a7c2-ada8fee016b2","mechanism_event_label":"The chlorinated derivative dampened one inflammatory output in cultured mouse cells.","subject":{"id":"0f99da38-0eae-53be-b1db-cb0146a11406","slug":"taurine-chloramine","display_name":"Taurine chloramine / TauCl","entity_type_key":"small_molecule"},"object":{"id":"700d037f-4b9b-534f-a644-14a6521312e8","slug":"mouse-taucl-macrophage-no","display_name":"Nitric oxide output from activated mouse RAW264.7 macrophages","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"021be326-ace8-57c5-a7c2-ada8fee016b2","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-taucl-no-event","event_type":"observed_relationship","label":"The chlorinated derivative dampened one inflammatory output in cultured mouse cells.","description":"Adding 0.5 mM taurine chloramine to activated RAW264.7 macrophages reduced measured nitrite output by 47%; cytosolic nitric-oxide-synthase activity was also inhibited by pretreatment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0f99da38-0eae-53be-b1db-cb0146a11406","slug":"taurine-chloramine","display_name":"Taurine chloramine / TauCl","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"700d037f-4b9b-534f-a644-14a6521312e8","slug":"mouse-taucl-macrophage-no","display_name":"Nitric oxide output from activated mouse RAW264.7 macrophages","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e423cd36-f183-522b-a5e4-5f9ef50eee7e","slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse RAW264.7 cells stimulated with interferon-gamma and LPS.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Millimolar derivative exposure does not establish the effect of oral taurine in humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Taurine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The chlorinated derivative dampened one inflammatory output in cultured mouse cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Taurine chloramine inhibits the synthesis of nitric oxide and the release of tumor necrosis factor in activated RAW 264.7 cells. · 1993 · https://pubmed.ncbi.nlm.nih.gov/7689627/ · DOI 10.1002/jlb.54.2.119","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"449d12f3-e7c6-5e2c-a211-37219abe3600","evidence_kind":"source_excerpt","locator":"Lines 369-375","start_line":369,"end_line":375,"excerpt":"## taurine-taucl-no\nThe chlorinated derivative dampened one inflammatory output in cultured mouse cells.\nAdding 0.5 mM taurine chloramine to activated RAW264.7 macrophages reduced measured nitrite output by 47%; cytosolic nitric-oxide-synthase activity was also inhibited by pretreatment.\nModel: Mouse RAW264.7 cells stimulated with interferon-gamma and LPS.\nLimitations: Millimolar derivative exposure does not establish the effect of oral taurine in humans.\nEvidence access: Primary abstract\nTaurine chloramine inhibits the synthesis of nitric oxide and the release of tumor necrosis factor in activated RAW 264.7 cells. · 1993 · https://pubmed.ncbi.nlm.nih.gov/7689627/ · DOI 10.1002/jlb.54.2.119","model_system":"Mouse RAW264.7 cells stimulated with interferon-gamma and LPS.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc8d9ab8-fdf9-5107-877c-336cf8005097","stable_key":"import-1acdb1e8-2159-5c7f-b39f-0153ff8024ae","title":"Taurine: synthesis, transport, mitochondrial decoding and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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