{"id":"fee007d9-6d95-529e-8250-92cf6ccdb6ed","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-sig-atra-rar-binding","predicate":"binds","statement":"All-trans RA bound the tested RAR alpha, beta and gamma preparations with high affinity.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"92d8a019-6ca9-56f5-8b6b-9bca3d74f042","mechanism_event_label":"The all-trans isomer directly engages RAR proteins.","subject":{"id":"05487b29-8c59-5af8-83cf-cf9c79c5ecb8","slug":"all-trans-retinoic-acid","display_name":"All-trans-retinoic acid","entity_type_key":"small_molecule"},"object":{"id":"0453bf0c-3c7c-5275-9b9e-8d7f01613b51","slug":"rar-family","display_name":"Retinoic acid receptor family","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"92d8a019-6ca9-56f5-8b6b-9bca3d74f042","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-sig-atra-rar-binding-event","event_type":"biochemical_relationship","label":"The all-trans isomer directly engages RAR proteins.","description":"All-trans RA bound the tested RAR alpha, beta and gamma preparations with high affinity.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ce392c9e-6483-5914-b18b-d6a2520dbbf6","slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"},"role":"nutrient-context","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"231bc87f-843e-57b3-ad84-06f63f656383","slug":"rara","display_name":"Retinoic acid receptor alpha","entity_type_key":"protein"},"role":"tested-subtype","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"1ab58d8f-c076-5814-ac44-1492046497ae","slug":"rarb","display_name":"Retinoic acid receptor beta","entity_type_key":"protein"},"role":"tested-subtype","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e3d36f8c-f454-5b21-8539-6e8f5eab3d8e","slug":"rarg","display_name":"Retinoic acid receptor gamma","entity_type_key":"protein"},"role":"tested-subtype","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"05487b29-8c59-5af8-83cf-cf9c79c5ecb8","slug":"all-trans-retinoic-acid","display_name":"All-trans-retinoic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"0453bf0c-3c7c-5275-9b9e-8d7f01613b51","slug":"rar-family","display_name":"Retinoic acid receptor family","entity_type_key":"protein_family"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_locator","value_text":"Abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Transfected COS-1 nucleosol; 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topical membership is not evidence of a direct dietary effect.\nplain_language: The all-trans isomer directly engages RAR proteins.\norganism: Recombinant receptors in African green monkey COS-1 cells\ntissue_or_cell_type: Nuclear extracts\nexperimental_model: Transfected COS-1 nucleosol; ligand competition and chimeric receptor reporters.\nlimitations: Binding affinity is not a dietary threshold.\nevidence_locator: Abstract\nexposure: Radioligand competition\n[va-allenby-1993] Retinoic acid receptors and retinoid X receptors: interactions with endogenous retinoic acids (1993). https://pubmed.ncbi.nlm.nih.gov/8380496/ DOI: 10.1073/pnas.90.1.30","model_system":"Transfected COS-1 nucleosol; ligand competition and chimeric receptor reporters.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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