{"id":"fedd8898-8480-5ce8-932b-a1ecf33abc8a","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-trans-pbmc-small-competition","predicate":"weakly_inhibits","statement":"Pantothenate at 10–1000 nmol/L reduced uptake of 475 pmol/L biotin by less than 12% in isolated human peripheral blood mononuclear cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"9b737229-3929-5c6a-a3e6-1d6742f653bf","mechanism_event_label":"At the tested physiological concentrations, B5 only slightly reduced biotin entry into these blood cells.","subject":{"id":"7bc62072-9517-5563-a3b8-8c5ab64a464b","slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"},"object":{"id":"7b199159-cc77-567c-ab7c-0b7bddceb6fa","slug":"human-pbmc-biotin-uptake","display_name":"Human peripheral blood mononuclear cell biotin uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"9b737229-3929-5c6a-a3e6-1d6742f653bf","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-trans-pbmc-small-competition-event","event_type":"biochemical_relationship","label":"At the tested physiological concentrations, B5 only slightly reduced biotin entry into these blood cells.","description":"Pantothenate at 10–1000 nmol/L reduced uptake of 475 pmol/L biotin by less than 12% in isolated human peripheral blood mononuclear cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"role":"competed_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7bc62072-9517-5563-a3b8-8c5ab64a464b","slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7b199159-cc77-567c-ab7c-0b7bddceb6fa","slug":"human-pbmc-biotin-uptake","display_name":"Human peripheral blood mononuclear cell biotin uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Isolated human peripheral blood mononuclear cells; radiolabeled biotin uptake and efflux assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"10–1000 nmol/L pantothenic acid with 475 pmol/L [3H]biotin.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-type-specific assay; the transporter was not molecularly identified as SLC5A6. This does not negate shared SMVT competition in other models and does not test high-dose supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"At the tested physiological concentrations, B5 only slightly reduced biotin entry into these blood cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-trans-pbmc1999] Human peripheral blood mononuclear cells: ; Inhibition of biotin transport by reversible competition with pantothenic acid is quantitatively minor. (1999). https://pubmed.ncbi.nlm.nih.gov/15539319/ DOI: 10.1016/s0955-2863(99)00024-8","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Isolated peripheral blood mononuclear cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"994e0011-04f6-5fe8-b355-58f2d39fe7b3","evidence_kind":"source_excerpt","locator":"Lines 457-468","start_line":457,"end_line":468,"excerpt":"### b5-trans-pbmc-small-competition\nPantothenate at 10–1000 nmol/L reduced uptake of 475 pmol/L biotin by less than 12% in isolated human peripheral blood mononuclear cells.\nCondition category: normal\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: At the tested physiological concentrations, B5 only slightly reduced biotin entry into these blood cells.\norganism: Homo sapiens\ntissue_or_cell_type: Isolated peripheral blood mononuclear cells\nexperimental_model: Isolated human peripheral blood mononuclear cells; radiolabeled biotin uptake and efflux assays\nlimitations: Cell-type-specific assay; the transporter was not molecularly identified as SLC5A6. This does not negate shared SMVT competition in other models and does not test high-dose supplementation.\nexposure: 10–1000 nmol/L pantothenic acid with 475 pmol/L [3H]biotin.\ncross_nutrient: true\n[b5-trans-pbmc1999] Human peripheral blood mononuclear cells: ; Inhibition of biotin transport by reversible competition with pantothenic acid is quantitatively minor. (1999). https://pubmed.ncbi.nlm.nih.gov/15539319/ DOI: 10.1016/s0955-2863(99)00024-8","model_system":"Isolated human peripheral blood mononuclear cells; radiolabeled biotin uptake and efflux assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-trans-pbmc1999] Human peripheral blood mononuclear cells: ; Inhibition of biotin transport by reversible competition with pantothenic acid is quantitatively minor. (1999). https://pubmed.ncbi.nlm.nih.gov/15539319/ DOI: 10.1016/s0955-2863(99)00024-8","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0716b500-dd0b-5425-b9d0-d88bc9c09e86","stable_key":"import-f992b796-377d-53bf-a39b-7e5d41dbe194","title":"Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b5a49f5a2373ca6064c9a4e014e052dad2f6f199a82bb09b26b82b831c36110b","revision_id":"78f3e793-f084-5ee2-8703-b661c4b650bf","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}