{"id":"fed92062-4446-5690-86b2-9078de7141e7","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-human-adipose-explant","predicate":"reduces","statement":"Human adipose explants exposed to 20 micromolar fisetin for 48 hours showed reduced senescence markers and selected secreted inflammatory factors.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"9f266934-c425-5c0f-87ab-300fa441f1dc","mechanism_event_label":"Human tissue responded outside the body.","subject":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"object":{"id":"4552cba2-dd41-59bf-bcb1-226f6a689fd8","slug":"human-adipose-explant-senescence-markers","display_name":"Human adipose-explant senescence and secretory markers","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"9f266934-c425-5c0f-87ab-300fa441f1dc","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-human-adipose-explant-event","event_type":"observed_relationship","label":"Human tissue responded outside the body.","description":"Human adipose explants exposed to 20 micromolar fisetin for 48 hours showed reduced senescence markers and selected secreted inflammatory factors.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4552cba2-dd41-59bf-bcb1-226f6a689fd8","slug":"human-adipose-explant-senescence-markers","display_name":"Human adipose-explant senescence and secretory markers","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Three biological explant replicates; washout before conditioned-medium collection.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Ex vivo exposure, not a human oral-treatment trial.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Human tissue responded outside the body.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Fisetin is a senotherapeutic that extends health and lifespan. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30279143/ · DOI 10.1016/j.ebiom.2018.09.015","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1562f771-598b-5003-88bc-dfa9e4d2ac7a","evidence_kind":"source_excerpt","locator":"Lines 216-222","start_line":216,"end_line":222,"excerpt":"## fisetin-human-adipose-explant\nHuman tissue responded outside the body.\nHuman adipose explants exposed to 20 micromolar fisetin for 48 hours showed reduced senescence markers and selected secreted inflammatory factors.\nModel: Three biological explant replicates; washout before conditioned-medium collection.\nLimitations: Ex vivo exposure, not a human oral-treatment trial.\nEvidence access: Primary full text\nFisetin is a senotherapeutic that extends health and lifespan. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30279143/ · DOI 10.1016/j.ebiom.2018.09.015","model_system":"Three biological explant replicates; washout before conditioned-medium collection.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c30e0764cd34bd062cfcbfaf32357ef29dfda30e7d4153c545178140f5054e09","revision_id":"40ff52c0-f4b9-5820-8abb-ff002c6364a4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}