{"id":"fec9f295-d5f4-5e66-afce-674f763cde0f","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-ryr-block","predicate":"inhibition_reduces","statement":"Ryanodine prevented resveratrol-induced AMPK/ACC phosphorylation in mouse C2C12 myotubes, supporting a ryanodine-receptor calcium-release route.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b02d3585-13bf-5502-8e4c-28a5f3647df2","mechanism_event_label":"Stored calcium release connects to the energy-sensing kinase.","subject":{"id":"9da4dee9-2aa1-58be-946b-c18b4b967fcb","slug":"mouse-ryr2","display_name":"Mouse ryanodine receptor 2 / Ryr2","entity_type_key":"protein"},"object":{"id":"871e74d6-6e9e-5e9c-9c6b-f269f59cb02a","slug":"mouse-ampk-complexes","display_name":"Mouse AMPK complexes","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"b02d3585-13bf-5502-8e4c-28a5f3647df2","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-ryr-block-event","event_type":"observed_relationship","label":"Stored calcium release connects to the energy-sensing kinase.","description":"Ryanodine prevented resveratrol-induced AMPK/ACC phosphorylation in mouse C2C12 myotubes, supporting a ryanodine-receptor calcium-release route.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9da4dee9-2aa1-58be-946b-c18b4b967fcb","slug":"mouse-ryr2","display_name":"Mouse ryanodine receptor 2 / Ryr2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"871e74d6-6e9e-5e9c-9c6b-f269f59cb02a","slug":"mouse-ampk-complexes","display_name":"Mouse AMPK complexes","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Pharmacological channel inhibition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not establish direct resveratrol binding to Ryr2.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Resveratrol collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Stored calcium release connects to the energy-sensing kinase.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Resveratrol ameliorates aging-related metabolic phenotypes by inhibiting cAMP phosphodiesterases. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22304913/ · DOI 10.1016/j.cell.2012.01.017","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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