{"id":"fe105bb0-dd75-5e51-a377-2996af2c08a0","stable_key":"475ea55a-65e9-51c3-a539-738a6a8f683f:mangiferin-norathyriol-ugt1a3","predicate":"competitively_inhibits","statement":"Norathyriol competitively inhibited UGT1A3; IC50 8.2 and Ki 1.6 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d77b0a7a-f4ad-5455-902b-611c37d54c9d","mechanism_event_label":"The aglycone has its own conjugation-enzyme interaction profile.","subject":{"id":"d0f7f42a-ac45-5144-9ea5-a7586896cc6e","slug":"norathyriol","display_name":"Norathyriol","entity_type_key":"small_molecule"},"object":{"id":"761185ae-f947-5d07-942b-6320d3d6e415","slug":"ugt1a3","display_name":"Human UDP-glucuronosyltransferase 1A3 / UGT1A3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"d77b0a7a-f4ad-5455-902b-611c37d54c9d","stable_key":"475ea55a-65e9-51c3-a539-738a6a8f683f:mangiferin-norathyriol-ugt1a3-event","event_type":"biochemical_relationship","label":"The aglycone has its own conjugation-enzyme interaction profile.","description":"Norathyriol competitively inhibited UGT1A3; IC50 8.2 and Ki 1.6 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d0f7f42a-ac45-5144-9ea5-a7586896cc6e","slug":"norathyriol","display_name":"Norathyriol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"761185ae-f947-5d07-942b-6320d3d6e415","slug":"ugt1a3","display_name":"Human UDP-glucuronosyltransferase 1A3 / UGT1A3","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/mangiferin-research/28621744.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e2741f9036a84104809f4d9813afeb1603b3e93e1d247ea0e4b5ff31be0e1ae5\", \"start_char\": 0, \"end_char\": 1562, \"text_sha256\": \"e2741f9036a84104809f4d9813afeb1603b3e93e1d247ea0e4b5ff31be0e1ae5\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant UGT inhibition kinetics","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Micromolar norathyriol; initial comparison at 100 micromolar","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Probe-dependent biochemical inhibition; no established human interaction threshold. Docking is not additional proof of binding in vivo.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Mangiferin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"mangiferin","display_name":"Mangiferin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Recombinant enzyme assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The aglycone has its own conjugation-enzyme interaction profile.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mangiferin-p28621744] In Vitro Comparative Study of the Inhibitory Effects of Mangiferin and Its Aglycone Norathyriol towards UDP-Glucuronosyl Transferase (UGT) Isoforms. (2017). https://pubmed.ncbi.nlm.nih.gov/28621744/ DOI: 10.3390/molecules22061008","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"4-methylumbelliferone glucuronidation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"fc76dc1c-3ba5-577f-ab69-e11fcc0c76c7","evidence_kind":"source_excerpt","locator":"Lines 874-885","start_line":874,"end_line":885,"excerpt":"### mangiferin-norathyriol-ugt1a3\nNorathyriol competitively inhibited UGT1A3; IC50 8.2 and Ki 1.6 micromolar.\nCondition category: normal\nnutrient_topic: Mangiferin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The aglycone has its own conjugation-enzyme interaction profile.\norganism: Recombinant enzyme assay\ntissue_or_cell_type: 4-methylumbelliferone glucuronidation\nexperimental_model: Recombinant UGT inhibition kinetics\nlimitations: Probe-dependent biochemical inhibition; no established human interaction threshold. Docking is not additional proof of binding in vivo.\nexposure: Micromolar norathyriol; initial comparison at 100 micromolar\nevidence_span: {\"source_cache\": \"artifacts/mangiferin-research/28621744.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e2741f9036a84104809f4d9813afeb1603b3e93e1d247ea0e4b5ff31be0e1ae5\", \"start_char\": 0, \"end_char\": 1562, \"text_sha256\": \"e2741f9036a84104809f4d9813afeb1603b3e93e1d247ea0e4b5ff31be0e1ae5\"}\n[mangiferin-p28621744] In Vitro Comparative Study of the Inhibitory Effects of Mangiferin and Its Aglycone Norathyriol towards UDP-Glucuronosyl Transferase (UGT) Isoforms. (2017). https://pubmed.ncbi.nlm.nih.gov/28621744/ DOI: 10.3390/molecules22061008","model_system":"Recombinant UGT inhibition kinetics","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mangiferin-p28621744] In Vitro Comparative Study of the Inhibitory Effects of Mangiferin and Its Aglycone Norathyriol towards UDP-Glucuronosyl Transferase (UGT) Isoforms. (2017). https://pubmed.ncbi.nlm.nih.gov/28621744/ DOI: 10.3390/molecules22061008","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"267f942a-695a-5b62-a858-f71c06321ff5","stable_key":"import-475ea55a-65e9-51c3-a539-738a6a8f683f","title":"Mangiferin: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"295cb3a13742b3caeed19347ffcbc7863f5372d983da1625a942428919b4dc98","revision_id":"d9c2ee89-ff22-5e86-9d7f-7cb7e17f9ed2","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}