{"id":"fe064d00-9a75-54ac-9740-1017236bae7b","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-aox-inhibition","predicate":"noncompetitively_inhibits","statement":"Thioridazine bound a distinct noncompetitive inhibitor site in human AOX1.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d968397b-86c6-5e31-9bdc-de9080cde864","mechanism_event_label":"A drug can inhibit AOX1 at a site separate from its substrate reaction.","subject":{"id":"54180b32-ac68-5a73-878a-39478bdfa7f1","slug":"thioridazine","display_name":"Thioridazine","entity_type_key":"small_molecule"},"object":{"id":"dcc4b976-aeb9-5dbd-8916-6507714db779","slug":"aox1","display_name":"Human aldehyde oxidase 1 / AOX1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"d968397b-86c6-5e31-9bdc-de9080cde864","stable_key":"dc8975b1-95ff-5d9b-be17-a1c04610cca7:mo-aox-inhibition-event","event_type":"biochemical_relationship","label":"A drug can inhibit AOX1 at a site separate from its substrate reaction.","description":"Thioridazine bound a distinct noncompetitive inhibitor site in human AOX1.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"54180b32-ac68-5a73-878a-39478bdfa7f1","slug":"thioridazine","display_name":"Thioridazine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"dcc4b976-aeb9-5dbd-8916-6507714db779","slug":"aox1","display_name":"Human aldehyde oxidase 1 / AOX1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/molybdenum-research/26322824.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0c289f1d166c7335431b4d041963accf22af348b4cac506145f2ccef59ca8188\", \"start_char\": 0, \"end_char\": 1067, \"text_sha256\": \"0c289f1d166c7335431b4d041963accf22af348b4cac506145f2ccef59ca8188\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human AOX1 structures and steady-state kinetics with substrate and inhibitor","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Phthalazine and thioridazine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Specific xenobiotic chemistry; does not establish broad detoxification benefits from molybdenum supplements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Molybdenum research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"molybdenum","display_name":"Molybdenum","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens protein","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A drug can inhibit AOX1 at a site separate from its substrate reaction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mo-p26322824] Structural insights into xenobiotic and inhibitor binding to human aldehyde oxidase. (2015). https://pubmed.ncbi.nlm.nih.gov/26322824/ DOI: 10.1038/nchembio.1895","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Recombinant purified AOX1","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"914d22f5-c10f-5d0e-b6a4-9689bd0c77e8","evidence_kind":"source_excerpt","locator":"Lines 846-857","start_line":846,"end_line":857,"excerpt":"### mo-aox-inhibition\nThioridazine bound a distinct noncompetitive inhibitor site in human AOX1.\nCondition category: normal\nnutrient_topic: Molybdenum research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A drug can inhibit AOX1 at a site separate from its substrate reaction.\norganism: Homo sapiens protein\ntissue_or_cell_type: Recombinant purified AOX1\nexperimental_model: Human AOX1 structures and steady-state kinetics with substrate and inhibitor\nlimitations: Specific xenobiotic chemistry; does not establish broad detoxification benefits from molybdenum supplements.\nexposure: Phthalazine and thioridazine\nevidence_span: {\"source_cache\": \"artifacts/molybdenum-research/26322824.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0c289f1d166c7335431b4d041963accf22af348b4cac506145f2ccef59ca8188\", \"start_char\": 0, \"end_char\": 1067, \"text_sha256\": \"0c289f1d166c7335431b4d041963accf22af348b4cac506145f2ccef59ca8188\"}\n[mo-p26322824] Structural insights into xenobiotic and inhibitor binding to human aldehyde oxidase. (2015). https://pubmed.ncbi.nlm.nih.gov/26322824/ DOI: 10.1038/nchembio.1895","model_system":"Human AOX1 structures and steady-state kinetics with substrate and inhibitor","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mo-p26322824] Structural insights into xenobiotic and inhibitor binding to human aldehyde oxidase. (2015). https://pubmed.ncbi.nlm.nih.gov/26322824/ DOI: 10.1038/nchembio.1895","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1aa6da60-8284-5252-8dd9-ac2250d5ced5","stable_key":"import-dc8975b1-95ff-5d9b-be17-a1c04610cca7","title":"Molybdenum: cofactor assembly, sulfur metabolism and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"143147eb9ac7fe750bdd33bb50d7773bc0cd49cc94faf219aebce3c3d760dd8f","revision_id":"90c2239d-290b-5e16-815a-f94d30569ebd","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}