{"id":"fdfd5a93-33a7-530e-a992-ade9cc8fa596","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-leucine-glucagon","predicate":"suppresses","statement":"Leucine suppressed alanine- and arginine-stimulated glucagon secretion in isolated human and mouse islets under the tested low-glucose conditions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"2f674157-b4dd-576f-9500-fce641df4252","mechanism_event_label":"One amino acid can reduce the hormone response to another.","subject":{"id":"f707c753-cc8c-5f06-90be-bb3d73c60a18","slug":"leucine","display_name":"L-Leucine","entity_type_key":"small_molecule"},"object":{"id":"91dfb576-701f-55e8-9fa2-2308bfc3cc9a","slug":"human-mouse-islet-alanine-glucagon","display_name":"Alanine-evoked glucagon in isolated human and mouse islets","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2f674157-b4dd-576f-9500-fce641df4252","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-leucine-glucagon-event","event_type":"observed_relationship","label":"One amino acid can reduce the hormone response to another.","description":"Leucine suppressed alanine- and arginine-stimulated glucagon secretion in isolated human and mouse islets under the tested low-glucose conditions.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f707c753-cc8c-5f06-90be-bb3d73c60a18","slug":"leucine","display_name":"L-Leucine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"91dfb576-701f-55e8-9fa2-2308bfc3cc9a","slug":"human-mouse-islet-alanine-glucagon","display_name":"Alanine-evoked glucagon in isolated human and mouse islets","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human and mouse islet perifusion; low-glucose amino-acid challenges.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Mixed-species functional observation, not a human supplementation trial; nutrient concentrations and glucose state determine interpretation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"One amino acid can reduce the hormone response to another.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Leucine Suppresses α-Cell cAMP and Glucagon Secretion via a Combination of Cell-Intrinsic and Islet Paracrine Signaling. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38870025/ · DOI 10.2337/db23-1013","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1dbfe650-e869-550c-bc7e-8e218739c73f","evidence_kind":"source_excerpt","locator":"Lines 344-350","start_line":344,"end_line":350,"excerpt":"## alanine-leucine-glucagon\nOne amino acid can reduce the hormone response to another.\nLeucine suppressed alanine- and arginine-stimulated glucagon secretion in isolated human and mouse islets under the tested low-glucose conditions.\nModel: Human and mouse islet perifusion; low-glucose amino-acid challenges.\nLimitations: Mixed-species functional observation, not a human supplementation trial; nutrient concentrations and glucose state determine interpretation.\nEvidence access: Primary abstract\nLeucine Suppresses α-Cell cAMP and Glucagon Secretion via a Combination of Cell-Intrinsic and Islet Paracrine Signaling. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38870025/ · DOI 10.2337/db23-1013","model_system":"Human and mouse islet perifusion; low-glucose amino-acid challenges.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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