{"id":"fdf22ffd-0dbf-5850-92d4-ff2fa80d999b","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-splice-map3k7a","predicate":"decreases_in_recorded_comparison","statement":"Apigenin decreased the relative abundance of Human MAP3K7-A transcript lacking exon 12 in MDA-MB-231 breast-cancer cells.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"d69e81c0-b6ed-55c0-b910-8bc56234099e","mechanism_event_label":"Apigenin decreased the relative abundance of Human MAP3K7-A transcript lacking exon 12 in MDA-MB-231 breast-cancer cells.","subject":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"object":{"id":"5f841318-574f-5fcf-aac1-893564729f5e","slug":"human-map3k7-a-transcript","display_name":"Human MAP3K7-A transcript lacking exon 12","entity_type_key":"rna"},"evidence_count":1,"mechanism_event":{"id":"d69e81c0-b6ed-55c0-b910-8bc56234099e","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-splice-map3k7a-event","event_type":"experimental_observation","label":"Apigenin decreased the relative abundance of Human MAP3K7-A transcript lacking exon 12 in MDA-MB-231 breast-cancer cells.","description":"### api-splice-map3k7a\n\nApigenin decreased the relative abundance of Human MAP3K7-A transcript lacking exon 12 in MDA-MB-231 breast-cancer cells.\nFigure 4. RNA isoform abundance is not automatically protein amount or proof that one isoform alone causes apoptosis.\nModel and exposure: 50 micromolar apigenin for 48 hours; RNA-seq and isoform-specific PCR.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Transcriptome reprogramming through alternative splicing triggered by apigenin drives cell death in triple-negative breast cancer. | 2023 | https://pubmed.ncbi.nlm.nih.gov/38092740/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC10719380/ | https://doi.org/10.1038/s41419-023-06342-6 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Apigenin","sequence_order":0,"notes":""},{"entity":{"id":"5f841318-574f-5fcf-aac1-893564729f5e","slug":"human-map3k7-a-transcript","display_name":"Human MAP3K7-A transcript lacking exon 12","entity_type_key":"rna"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"852a7b7e-532b-51e6-bb1a-de63dac60035","slug":"human-hnrnpa2b1","display_name":"Human heterogeneous nuclear ribonucleoprotein A2/B1 / HNRNPA2B1","entity_type_key":"protein"},"role":"splicing factor implicated by prior binding and enrichment","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4933f605-b67f-58af-ad7c-65f887fcf82a","slug":"human-map3k7-b-transcript","display_name":"Human MAP3K7-B transcript containing exon 12","entity_type_key":"rna"},"role":"increased alternative isoform","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Apigenin\", \"comparator\": \"DMSO\", \"endpoint\": \"Apigenin decreased the relative abundance of Human MAP3K7-A transcript lacking exon 12 in MDA-MB-231 breast-cancer cells.\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"50 micromolar apigenin for 48 hours; RNA-seq and isoform-specific PCR.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Apigenin decreased the relative abundance of Human MAP3K7-A transcript lacking exon 12 in MDA-MB-231 breast-cancer cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Transcriptome reprogramming through alternative splicing triggered by apigenin drives cell death in triple-negative breast cancer. | https://pubmed.ncbi.nlm.nih.gov/38092740/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC10719380/ | https://doi.org/10.1038/s41419-023-06342-6","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-splice-map3k7a; Figure 4. RNA isoform abundance is not automatically protein amount or proof that one isoform alone causes apoptosis.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3210529b-e446-583e-9e42-c683daae48e8","evidence_kind":"source_excerpt","locator":"Lines 660-666","start_line":660,"end_line":666,"excerpt":"### api-splice-map3k7a\n\nApigenin decreased the relative abundance of Human MAP3K7-A transcript lacking exon 12 in MDA-MB-231 breast-cancer cells.\nFigure 4. RNA isoform abundance is not automatically protein amount or proof that one isoform alone causes apoptosis.\nModel and exposure: 50 micromolar apigenin for 48 hours; RNA-seq and isoform-specific PCR.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Transcriptome reprogramming through alternative splicing triggered by apigenin drives cell death in triple-negative breast cancer. | 2023 | https://pubmed.ncbi.nlm.nih.gov/38092740/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC10719380/ | https://doi.org/10.1038/s41419-023-06342-6 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","model_system":"50 micromolar apigenin for 48 hours; RNA-seq and isoform-specific PCR.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}