{"id":"fd94cc55-b947-50cd-a81f-a829214884c5","stable_key":"ea2f0904-ab2c-5d1e-a3d4-3f7ecdd70cd6:caf-ip3","predicate":"inhibits","statement":"Caffeine inhibited IP3-gated channels with half-inhibition at 1.64 mM; 5 mM reduced opening frequency more than threefold without changing conductance.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"9d629489-8ee4-5454-8a04-31cb2dd9921a","mechanism_event_label":"Caffeine can inhibit one calcium-release channel while activating another.","subject":{"id":"ef028472-7c33-583d-b52e-40676004d370","slug":"caffeine","display_name":"Caffeine","entity_type_key":"small_molecule"},"object":{"id":"8813a26f-48da-5cfa-a526-cd0e2df17afb","slug":"canine-cerebellar-ip3-receptor","display_name":"Canine cerebellar IP3-gated calcium channel preparation","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"9d629489-8ee4-5454-8a04-31cb2dd9921a","stable_key":"ea2f0904-ab2c-5d1e-a3d4-3f7ecdd70cd6:caf-ip3-event","event_type":"observed_relationship","label":"Caffeine can inhibit one calcium-release channel while activating another.","description":"Caffeine inhibited IP3-gated channels with half-inhibition at 1.64 mM; 5 mM reduced opening frequency more than threefold without changing conductance.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ef028472-7c33-583d-b52e-40676004d370","slug":"caffeine","display_name":"Caffeine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8813a26f-48da-5cfa-a526-cd0e2df17afb","slug":"canine-cerebellar-ip3-receptor","display_name":"Canine cerebellar IP3-gated calcium channel preparation","entity_type_key":"protein_family"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a2a336cb-a3da-5a89-b360-b0326a768ff5","slug":"ip3-gated-calcium-current","display_name":"IP3-gated intracellular calcium-channel current","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Canine cerebellar channels in planar lipid bilayers.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Millimolar concentrations; opposite channel effects are distinct targets, not a scientific contradiction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Caffeine collection; salts, coffee, species and coexposure contexts retain their identities.","comparator":null,"unit":null,"notes":"","entity":{"slug":"caffeine","display_name":"Caffeine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Caffeine can inhibit one calcium-release channel while activating another.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Caffeine-induced inhibition of inositol(1,4,5)-trisphosphate-gated calcium channels from cerebellum. · 1994 · https://pubmed.ncbi.nlm.nih.gov/8186468/ · DOI 10.1091/mbc.5.1.97","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c1c07788-0ee9-5cf9-bca2-e763b755a77b","evidence_kind":"source_excerpt","locator":"Lines 388-394","start_line":388,"end_line":394,"excerpt":"## caf-ip3\nCaffeine can inhibit one calcium-release channel while activating another.\nCaffeine inhibited IP3-gated channels with half-inhibition at 1.64 mM; 5 mM reduced opening frequency more than threefold without changing conductance.\nModel: Canine cerebellar channels in planar lipid bilayers.\nLimitations: Millimolar concentrations; opposite channel effects are distinct targets, not a scientific contradiction.\nEvidence access: Primary abstract\nCaffeine-induced inhibition of inositol(1,4,5)-trisphosphate-gated calcium channels from cerebellum. · 1994 · https://pubmed.ncbi.nlm.nih.gov/8186468/ · DOI 10.1091/mbc.5.1.97","model_system":"Canine cerebellar channels in planar lipid bilayers.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access stated with the claim.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"40a654db-878c-5471-bc6c-88f4f52599c0","stable_key":"import-ea2f0904-ab2c-5d1e-a3d4-3f7ecdd70cd6","title":"Caffeine: receptors, metabolism, nutrient interactions, adaptation and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"780d73d4abd45a0284d2cc0c646dbe8432ae347362891a4b98a19a61341a7325","revision_id":"f36ffb0a-d6df-522f-b783-5e30214b9e26","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}