{"id":"fd16d275-9e3a-5d03-bbdb-4eac8245e218","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-mouse-micronuclei","predicate":"does_not_significantly_increase","statement":"CHD-FA did not significantly increase micronucleated polychromatic erythrocytes in tested mice.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"131c2d36-744d-5614-b555-8b2127a988dc","mechanism_event_label":"The mouse chromosomal-damage endpoint was negative.","subject":{"id":"5184fd06-5a8e-5e9d-99ec-c2feaa59ccde","slug":"carbohydrate-derived-fulvic-acid","display_name":"Carbohydrate-derived fulvic acid / CHD-FA (preparation class)","entity_type_key":"chemical_species"},"object":{"id":"d7d6f2ee-e167-5205-a642-06a1299d71bd","slug":"mouse-chdfa-micronuclei","display_name":"Mouse erythrocyte micronuclei after CHD-FA","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"131c2d36-744d-5614-b555-8b2127a988dc","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-mouse-micronuclei-event","event_type":"observed_relationship","label":"The mouse chromosomal-damage endpoint was negative.","description":"CHD-FA did not significantly increase micronucleated polychromatic erythrocytes in tested mice.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5184fd06-5a8e-5e9d-99ec-c2feaa59ccde","slug":"carbohydrate-derived-fulvic-acid","display_name":"Carbohydrate-derived fulvic acid / CHD-FA (preparation class)","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d7d6f2ee-e167-5205-a642-06a1299d71bd","slug":"mouse-chdfa-micronuclei","display_name":"Mouse erythrocyte micronuclei after CHD-FA","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0aa6c0fc-8374-56e0-b172-6c40b6366d21","slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Male mouse micronucleus assay; reported doses up to 100 mg/kg, sampling at 24/48 hours.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The 2026 study was electronically published June 21, with a December issue date. Negative results in one assay do not erase positive or uncertain findings in another; chronic exposure remains unresolved. Full results report reduced PCE/NCE at 100 mg/kg after 48 hours despite the abstract summary; retain this marrow-response finding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fulvic acid collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"The mouse chromosomal-damage endpoint was negative.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Genotoxicity of Carbohydrate Derived Fulvic Acid. · 2026 · https://pubmed.ncbi.nlm.nih.gov/42383226/ · DOI 10.1016/j.toxrep.2026.102298","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"86a43059-db9b-5d49-9a31-cdc114940fa0","evidence_kind":"source_excerpt","locator":"Lines 300-306","start_line":300,"end_line":306,"excerpt":"## fulvic-acid-mouse-micronuclei\nThe mouse chromosomal-damage endpoint was negative.\nCHD-FA did not significantly increase micronucleated polychromatic erythrocytes in tested mice.\nModel: Male mouse micronucleus assay; reported doses up to 100 mg/kg, sampling at 24/48 hours.\nLimitations: The 2026 study was electronically published June 21, with a December issue date. Negative results in one assay do not erase positive or uncertain findings in another; chronic exposure remains unresolved. Full results report reduced PCE/NCE at 100 mg/kg after 48 hours despite the abstract summary; retain this marrow-response finding.\nEvidence access: Primary full text\nGenotoxicity of Carbohydrate Derived Fulvic Acid. · 2026 · https://pubmed.ncbi.nlm.nih.gov/42383226/ · DOI 10.1016/j.toxrep.2026.102298","model_system":"Male mouse micronucleus assay; reported doses up to 100 mg/kg, sampling at 24/48 hours.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b00a1818-46e4-52cc-8dfe-a647d5c08ed7","stable_key":"import-788beb7b-7928-5f89-83d4-5556abf3702c","title":"Fulvic acid: mixture identity, mineral chemistry, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"52c2c3ee6bb23d943a40e1c39dd9de5cca7acd6efa72479cb824149e85cc7968","revision_id":"94dfc01b-c93e-55ff-8f48-bb2e9a1979f6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}