{"id":"fcf6e356-7745-5d9d-b668-00deb3550599","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-nqo2-binding","predicate":"binds_and_inhibits","statement":"Resveratrol bound the active-site cleft of human NQO2 beside FAD, with reported dissociation constant 35 nM, and inhibited activity in vitro.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"136a28bf-f011-53d4-be38-157f6a45fe80","mechanism_event_label":"A riboflavin-derived cofactor sits beside a directly observed binding site.","subject":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"object":{"id":"1c48fce8-58ed-5238-bdfc-6ce3924f20a7","slug":"nqo2","display_name":"Human quinone reductase 2 / NQO2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"136a28bf-f011-53d4-be38-157f6a45fe80","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-nqo2-binding-event","event_type":"observed_relationship","label":"A riboflavin-derived cofactor sits beside a directly observed binding site.","description":"Resveratrol bound the active-site cleft of human NQO2 beside FAD, with reported dissociation constant 35 nM, and inhibited activity in vitro.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1c48fce8-58ed-5238-bdfc-6ce3924f20a7","slug":"nqo2","display_name":"Human quinone reductase 2 / NQO2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e2cd7179-f218-54e8-9ce9-7a836ae35fac","slug":"fad","display_name":"FAD","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified enzyme crystal structure and biochemical assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Binding beside FAD does not mean resveratrol depletes riboflavin or displaces FAD.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Resveratrol collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A riboflavin-derived cofactor sits beside a directly observed binding site.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Crystal structure of quinone reductase 2 in complex with resveratrol. · 2004 · https://pubmed.ncbi.nlm.nih.gov/15350128/ · DOI 10.1021/bi049162o","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e723a961-26c0-5163-b629-4091ad960606","evidence_kind":"source_excerpt","locator":"Lines 110-116","start_line":110,"end_line":116,"excerpt":"## resveratrol-nqo2-binding\nA riboflavin-derived cofactor sits beside a directly observed binding site.\nResveratrol bound the active-site cleft of human NQO2 beside FAD, with reported dissociation constant 35 nM, and inhibited activity in vitro.\nModel: Purified enzyme crystal structure and biochemical assays.\nLimitations: Binding beside FAD does not mean resveratrol depletes riboflavin or displaces FAD.\nEvidence access: Primary abstract\nCrystal structure of quinone reductase 2 in complex with resveratrol. · 2004 · https://pubmed.ncbi.nlm.nih.gov/15350128/ · DOI 10.1021/bi049162o","model_system":"Purified enzyme crystal structure and biochemical assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2844eda9-a122-5357-ae47-cd58227c5994","stable_key":"import-52871722-4aa6-5453-a902-3e76356db327","title":"Resveratrol: metabolites, target selectivity and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"04885b4bee72f912a7f3f618f1b2bf79c944dbcb20500398c2729fc8228dcd9a","revision_id":"99504ff2-f1d3-5f4c-bc31-e1030d27a918","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}