{"id":"fcb89d0c-0706-5c35-9a97-c672e9e8985c","stable_key":"649e861b-265a-5912-bc36-3a73e53ef892:l-serine-psph-final-step","predicate":"dephosphorylates_to","statement":"Human phosphoserine phosphatase catalyzes the final, effectively irreversible dephosphorylation step of the phosphorylated serine synthesis pathway.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a5ce3bda-2e1e-531c-a8f1-9bcbe8285b91","mechanism_event_label":"The last enzyme releases free serine from its phosphorylated precursor.","subject":{"id":"1177919d-d7f4-5272-b40c-444f5dbee4d2","slug":"psph","display_name":"Human phosphoserine phosphatase / PSPH","entity_type_key":"protein"},"object":{"id":"c882cd0b-9d83-5b07-bd1c-fe4d45657dcb","slug":"l-serine","display_name":"L-Serine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"a5ce3bda-2e1e-531c-a8f1-9bcbe8285b91","stable_key":"649e861b-265a-5912-bc36-3a73e53ef892:l-serine-psph-final-step-event","event_type":"observed_relationship","label":"The last enzyme releases free serine from its phosphorylated precursor.","description":"Human phosphoserine phosphatase catalyzes the final, effectively irreversible dephosphorylation step of the phosphorylated serine synthesis pathway.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1177919d-d7f4-5272-b40c-444f5dbee4d2","slug":"psph","display_name":"Human phosphoserine phosphatase / PSPH","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c882cd0b-9d83-5b07-bd1c-fe4d45657dcb","slug":"l-serine","display_name":"L-Serine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f8d93623-1546-5bb5-a8a8-55761070bf49","slug":"o-phospho-l-serine","display_name":"O-Phospho-L-serine, free metabolite","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human PSPH functional analysis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Free phosphoserine is distinct from a phosphorylated serine residue in a protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Serine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-serine","display_name":"L-Serine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The last enzyme releases free serine from its phosphorylated precursor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Effect of l-serine and magnesium ions on the functional properties of human phosphoserine phosphatase and its pathogenetic variants. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38278334/ · DOI 10.1016/j.bbadis.2024.167034","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3dce09c4-da57-5290-8c35-5a7bf33f2646","evidence_kind":"source_excerpt","locator":"Lines 46-52","start_line":46,"end_line":52,"excerpt":"## l-serine-psph-final-step\nThe last enzyme releases free serine from its phosphorylated precursor.\nHuman phosphoserine phosphatase catalyzes the final, effectively irreversible dephosphorylation step of the phosphorylated serine synthesis pathway.\nModel: Recombinant human PSPH functional analysis.\nLimitations: Free phosphoserine is distinct from a phosphorylated serine residue in a protein.\nEvidence access: Primary abstract\nEffect of l-serine and magnesium ions on the functional properties of human phosphoserine phosphatase and its pathogenetic variants. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38278334/ · DOI 10.1016/j.bbadis.2024.167034","model_system":"Recombinant human PSPH functional analysis.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"70bcf57a-36bb-563f-9073-2d616e5f155a","stable_key":"import-649e861b-265a-5912-bc36-3a73e53ef892","title":"L-Serine: synthesis, one-carbon metabolism, lipids and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"22a750ce2b94f8607268c58322c48c4f0468f403b72a4008307538a6e5ff86d5","revision_id":"c1d6a7e1-9558-5bc6-9e3b-7080c26d3dbc","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}