{"id":"fcb13d71-5548-5bd9-b33e-f5e7e9aa496c","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-inpp1-calcium-manganese","predicate":"inhibits","statement":"Calcium and manganese each inhibited the calf-brain enzyme by about half near 6 micromolar under the reported assay conditions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"59be8ca3-9fed-5f30-8f64-0d9d7fc51573","mechanism_event_label":"A mineral that activates one enzyme can inhibit another.","subject":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"object":{"id":"49a701d3-dc96-5389-bafd-6948f38dc393","slug":"bovine-inpp1","display_name":"Bovine inositol polyphosphate 1-phosphatase / INPP1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"59be8ca3-9fed-5f30-8f64-0d9d7fc51573","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-inpp1-calcium-manganese-event","event_type":"observed_relationship","label":"A mineral that activates one enzyme can inhibit another.","description":"Calcium and manganese each inhibited the calf-brain enzyme by about half near 6 micromolar under the reported assay conditions.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"49a701d3-dc96-5389-bafd-6948f38dc393","slug":"bovine-inpp1","display_name":"Bovine inositol polyphosphate 1-phosphatase / INPP1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8e2e3902-5e26-52e3-9c85-1d7c0ace1637","slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"a8082b11-c484-5792-bb81-48e8e699cbe4","slug":"manganese-ion","display_name":"Mn2+","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified calf-brain enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Free-ion assay concentrations do not establish dietary antagonism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lithium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"A mineral that activates one enzyme can inhibit another.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inositol polyphosphate 1-phosphatase from calf brain. Purification and inhibition by Li+, Ca2+, and Mn2+. · 1987 · https://pubmed.ncbi.nlm.nih.gov/2824473/","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a290453d-d8d0-5f8a-8524-f2b85ba6959a","evidence_kind":"source_excerpt","locator":"Lines 64-70","start_line":64,"end_line":70,"excerpt":"## lithium-inpp1-calcium-manganese\nA mineral that activates one enzyme can inhibit another.\nCalcium and manganese each inhibited the calf-brain enzyme by about half near 6 micromolar under the reported assay conditions.\nModel: Purified calf-brain enzyme.\nLimitations: Free-ion assay concentrations do not establish dietary antagonism.\nEvidence access: Primary abstract\nInositol polyphosphate 1-phosphatase from calf brain. Purification and inhibition by Li+, Ca2+, and Mn2+. · 1987 · https://pubmed.ncbi.nlm.nih.gov/2824473/","model_system":"Purified calf-brain enzyme.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b2dd2f3c-5c4c-5a12-9a0d-91b521ffcf98","stable_key":"import-b7798a90-72a3-5454-a321-c4be88bf0cc4","title":"Lithium: metal-sensitive enzymes, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"dc67e965ba78458cf2d88d000d687641d9104354115ab16529a3a64941bd777d","revision_id":"4af127bf-9ccc-516b-9481-fafd453c2c35","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}