{"id":"fc8bd67f-6825-5b45-80a2-cc0fa75001d6","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-pkcb-junction","predicate":"modulates","statement":"Sodium butyrate increased PKC-beta2 Ser660 phosphorylation; PKC-beta inhibition blocked the promoted junction reassembly.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"47c59eaf-4c8c-5fcb-8a15-b5a34e9f86d2","mechanism_event_label":"A downstream kinase contributed to rebuilding the junction.","subject":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"object":{"id":"505495d2-667a-507e-9446-3e58c4f7aec2","slug":"human-prkcb-beta2","display_name":"Human protein kinase C beta II / PRKCB beta2 isoform","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"47c59eaf-4c8c-5fcb-8a15-b5a34e9f86d2","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-pkcb-junction-event","event_type":"observed_relationship","label":"A downstream kinase contributed to rebuilding the junction.","description":"Sodium butyrate increased PKC-beta2 Ser660 phosphorylation; PKC-beta inhibition blocked the promoted junction reassembly.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"505495d2-667a-507e-9446-3e58c4f7aec2","slug":"human-prkcb-beta2","display_name":"Human protein kinase C beta II / PRKCB beta2 isoform","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bf38462d-0c44-58c8-a619-befacd80fe54","slug":"human-butyrate-tight-junction-assembly","display_name":"Human Caco-2 tight-junction assembly after butyrate","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4baf6011-dcc4-5a69-9c77-a21de6060383","slug":"sodium-butyrate","display_name":"Sodium butyrate","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human Caco-2 signaling and inhibitor experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Inhibitor sensitivity does not imply direct binding of butyrate to PKC-beta2.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Butyrate collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A downstream kinase contributed to rebuilding the junction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Sodium Butyrate Promotes Reassembly of Tight Junctions in Caco-2 Monolayers Involving Inhibition of MLCK/MLC2 Pathway and Phosphorylation of PKCβ2. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27735862/ · DOI 10.3390/ijms17101696","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1e8f9815-38b0-53ce-b1ad-4c85a0c064d0","evidence_kind":"source_excerpt","locator":"Lines 278-284","start_line":278,"end_line":284,"excerpt":"## butyrate-pkcb-junction\nA downstream kinase contributed to rebuilding the junction.\nSodium butyrate increased PKC-beta2 Ser660 phosphorylation; PKC-beta inhibition blocked the promoted junction reassembly.\nModel: Human Caco-2 signaling and inhibitor experiments.\nLimitations: Inhibitor sensitivity does not imply direct binding of butyrate to PKC-beta2.\nEvidence access: Primary abstract\nSodium Butyrate Promotes Reassembly of Tight Junctions in Caco-2 Monolayers Involving Inhibition of MLCK/MLC2 Pathway and Phosphorylation of PKCβ2. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27735862/ · DOI 10.3390/ijms17101696","model_system":"Human Caco-2 signaling and inhibitor experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"33205bb8-dcbe-5d2c-afca-86075d7748d1","stable_key":"import-104835a3-b891-5dc4-a6bb-42ab7c6f96c4","title":"Butyrate: microbial production, fuel use, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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