{"id":"fc805c91-6092-59af-90be-8d99141dcc2e","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-diclofenac-oat3","predicate":"inhibits","statement":"Diclofenac inhibited OAT3-mediated steviol-glucuronide uptake, with IC50 8.0 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"57fc1609-a912-546f-995f-ff83282c5640","mechanism_event_label":"The same drug intersects two different clearance steps.","subject":{"id":"2d3faead-6df2-5e17-ad25-6f7c20a2ae05","slug":"diclofenac","display_name":"Diclofenac","entity_type_key":"drug"},"object":{"id":"5e4ab3f9-5b15-59f2-94e6-24ca9bda87bb","slug":"human-oat3-steviol-glucuronide-uptake","display_name":"Human OAT3-mediated steviol-glucuronide uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"57fc1609-a912-546f-995f-ff83282c5640","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-diclofenac-oat3-event","event_type":"observed_relationship","label":"The same drug intersects two different clearance steps.","description":"Diclofenac inhibited OAT3-mediated steviol-glucuronide uptake, with IC50 8.0 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2d3faead-6df2-5e17-ad25-6f7c20a2ae05","slug":"diclofenac","display_name":"Diclofenac","entity_type_key":"drug"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5e4ab3f9-5b15-59f2-94e6-24ca9bda87bb","slug":"human-oat3-steviol-glucuronide-uptake","display_name":"Human OAT3-mediated steviol-glucuronide uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c78086c3-7657-589b-b353-1691df2d9f8f","slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4606352d-ad2e-580f-87eb-e0ccf0d02fd9","slug":"slc22a8","display_name":"Human organic anion transporter 3 / SLC22A8","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"f008b74d-164b-5663-a72e-04a48fe7a8fb","slug":"steviol-glucuronide","display_name":"Steviol glucuronide","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"In vitro uptake assay; distinct from the glucuronidation experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Combining the two records does not quantify a net in vivo interaction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Stevia collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The same drug intersects two different clearance steps.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Transmembrane transport of steviol glucuronide and its potential interaction with selected drugs and natural compounds. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26525112/ · DOI 10.1016/j.fct.2015.10.011","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"44144033-c741-5fb7-a3c8-b0cd64884de1","evidence_kind":"source_excerpt","locator":"Lines 138-144","start_line":138,"end_line":144,"excerpt":"## stevia-diclofenac-oat3\nThe same drug intersects two different clearance steps.\nDiclofenac inhibited OAT3-mediated steviol-glucuronide uptake, with IC50 8.0 micromolar.\nModel: In vitro uptake assay; distinct from the glucuronidation experiment.\nLimitations: Combining the two records does not quantify a net in vivo interaction.\nEvidence access: Primary abstract\nTransmembrane transport of steviol glucuronide and its potential interaction with selected drugs and natural compounds. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26525112/ · DOI 10.1016/j.fct.2015.10.011","model_system":"In vitro uptake assay; distinct from the glucuronidation experiment.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"295304c9-609c-5b05-a18f-a00ab7b965ed","stable_key":"import-6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7","title":"Stevia: glycoside metabolism, taste, ion channels and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"a36a82c2f8a0a971ea43e9e742db65f45848d13ab77310c0dd6b5cd55e31fc16","revision_id":"ea0355af-a926-511a-a7f2-1750baa95a8d","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}