{"id":"fc7a4130-6730-5db6-b486-c13d8bce4c52","stable_key":"e13b03e1-a614-543a-ac1b-97df18cfe30d:iron-high-iron-copper-deficiency","predicate":"excess_caused","statement":"Very-high-iron feeding caused low serum/tissue copper, reduced circulating ceruloplasmin activity, anemia and cardiac hypertrophy in the studied rats.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"9a77033e-b4f8-5b04-8b59-8f27cd4099e1","mechanism_event_label":"Anemia developed despite abundant iron because another part of iron handling became deficient.","subject":{"id":"89bcaf42-b4ab-5760-8c2e-44eace10cee0","slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"},"object":{"id":"3ca86e56-991e-532b-b8be-c1445d560949","slug":"rat-copper-deficiency-anemia","display_name":"Anemia in copper-deficient rats","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"9a77033e-b4f8-5b04-8b59-8f27cd4099e1","stable_key":"e13b03e1-a614-543a-ac1b-97df18cfe30d:iron-high-iron-copper-deficiency-event","event_type":"biochemical_relationship","label":"Anemia developed despite abundant iron because another part of iron handling became deficient.","description":"Very-high-iron feeding caused low serum/tissue copper, reduced circulating ceruloplasmin activity, anemia and cardiac hypertrophy in the studied rats.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9f0afdde-1ec1-5c8a-bb5e-f3b2b75f67f6","slug":"copper","display_name":"Copper","entity_type_key":"nutrient_element"},"role":"depleted_nutrient","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1a466d94-c94f-5611-b0b4-f48f975cd641","slug":"rat-cp","display_name":"Rat ceruloplasmin / Cp","entity_type_key":"protein"},"role":"copper_dependent_enzyme","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"89bcaf42-b4ab-5760-8c2e-44eace10cee0","slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"3ca86e56-991e-532b-b8be-c1445d560949","slug":"rat-copper-deficiency-anemia","display_name":"Anemia in copper-deficient rats","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/iron-research/27537180.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"59f2a83f1bffcacfed0df109f3690413aa2e6f08be6ad400817c5cbe2165e523\", \"start_char\": 0, \"end_char\": 1840, \"text_sha256\": \"59f2a83f1bffcacfed0df109f3690413aa2e6f08be6ad400817c5cbe2165e523\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Five-week factorial iron/copper feeding","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Approximately 8800, 80 or 11 ppm iron crossed with approximately 183, 8 or 0.9 ppm copper","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Very high experimental iron exposure; not an estimate of human supplement risk at ordinary intake. Copper restoration did not normalize every iron measure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iron research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Weanling male Sprague-Dawley rats","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Anemia developed despite abundant iron because another part of iron handling became deficient.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[iron-p27537180] High-Iron Consumption Impairs Growth and Causes Copper-Deficiency Anemia in Weanling Sprague-Dawley Rats. (2016). https://pubmed.ncbi.nlm.nih.gov/27537180/ DOI: 10.1371/journal.pone.0161033","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Systemic copper and iron status","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"289e6bfe-ec87-585e-ae3f-e79919dc50fa","evidence_kind":"source_excerpt","locator":"Lines 914-925","start_line":914,"end_line":925,"excerpt":"### iron-high-iron-copper-deficiency\nVery-high-iron feeding caused low serum/tissue copper, reduced circulating ceruloplasmin activity, anemia and cardiac hypertrophy in the studied rats.\nCondition category: nutrient_deficiency\nnutrient_topic: Iron research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Anemia developed despite abundant iron because another part of iron handling became deficient.\norganism: Weanling male Sprague-Dawley rats\ntissue_or_cell_type: Systemic copper and iron status\nexperimental_model: Five-week factorial iron/copper feeding\nlimitations: Very high experimental iron exposure; not an estimate of human supplement risk at ordinary intake. Copper restoration did not normalize every iron measure.\nexposure: Approximately 8800, 80 or 11 ppm iron crossed with approximately 183, 8 or 0.9 ppm copper\nevidence_span: {\"source_cache\": \"artifacts/iron-research/27537180.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"59f2a83f1bffcacfed0df109f3690413aa2e6f08be6ad400817c5cbe2165e523\", \"start_char\": 0, \"end_char\": 1840, \"text_sha256\": \"59f2a83f1bffcacfed0df109f3690413aa2e6f08be6ad400817c5cbe2165e523\"}\n[iron-p27537180] High-Iron Consumption Impairs Growth and Causes Copper-Deficiency Anemia in Weanling Sprague-Dawley Rats. (2016). https://pubmed.ncbi.nlm.nih.gov/27537180/ DOI: 10.1371/journal.pone.0161033","model_system":"Five-week factorial iron/copper feeding","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [iron-p27537180] High-Iron Consumption Impairs Growth and Causes Copper-Deficiency Anemia in Weanling Sprague-Dawley Rats. (2016). https://pubmed.ncbi.nlm.nih.gov/27537180/ DOI: 10.1371/journal.pone.0161033","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1560864c-91d1-58dc-90c4-49bf61aba3de","stable_key":"import-e13b03e1-a614-543a-ac1b-97df18cfe30d","title":"Iron: absorption, trafficking, iron-dependent enzymes and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f9dab767a21c9bbfef3227707fe3c82953536bb1335599dd4ef93b5ea2dbf03f","revision_id":"77700d7f-2902-54b9-adae-8cbc77b24682","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}