{"id":"fc76e85f-6900-5894-8d5c-51d17cbe760f","stable_key":"35ec55a7-323c-5c28-979e-3bdafe9d5769:mixture-reconstruction","predicate":"is_exposure_origin_for_reconstructed_mixture","statement":"The tested signatures reconstruct serum metabolite profiles measured after labeled C3G exposure; they are separate mixtures, not intact C3G.","claim_class":"identity","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"65b42e27-74a7-5471-8247-74c9a94210e0","mechanism_event_label":"The tested signatures reconstruct serum metabolite profiles measured after labeled C3G exposure; they are separate mixtures, not intact C3G.","subject":{"id":"75149db0-02f7-5d32-8c7a-5c111d90d8ee","slug":"cyanidin-3-glucoside","display_name":"Cyanidin 3-O-beta-D-glucopyranoside","entity_type_key":"small_molecule"},"object":{"id":"415608c3-d57b-5b17-aa69-c19e547330b9","slug":"c3g-human-serum-metabolite-signatures","display_name":"Reconstructed 1-, 6- and 24-hour human C3G metabolite signatures","entity_type_key":"chemical_species"},"evidence_count":1,"mechanism_event":{"id":"65b42e27-74a7-5471-8247-74c9a94210e0","stable_key":"35ec55a7-323c-5c28-979e-3bdafe9d5769:mixture-reconstruction-event","event_type":"experimental_preparation_provenance","label":"The tested signatures reconstruct serum metabolite profiles measured after labeled C3G exposure; they are separate mixtures, not intact C3G.","description":"**Low-concentration mixtures can change VCAM-1 without a universal IL-6 effect.** Warner's 2017 study reconstructed the tracer cohort's 1-, 6- and 24-hour serum signatures. Mixtures reduced TNF-stimulated VCAM-1; some effects remained at total concentrations of 0.19–0.44 µM. IL-6 secretion decreased in HUVECs at selected higher mixture concentrations, but not detectably in HCAECs. TNF stimulation also differed between the cell types. Figure 3 preserves this important cell-specific null. Phosphorylated NF-κB p65 did not significantly change in the tested HCAEC experiment. These are mixture findings, not proof that PCA alone suppresses NF-κB at dietary exposure. [Warner et al., 2017, Figures 2–5](https://pmc.ncbi.nlm.nih.gov/articles/PMC5600085/).","status":"provisional","compartment":null,"participants":[{"entity":{"id":"75149db0-02f7-5d32-8c7a-5c111d90d8ee","slug":"cyanidin-3-glucoside","display_name":"Cyanidin 3-O-beta-D-glucopyranoside","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"415608c3-d57b-5b17-aa69-c19e547330b9","slug":"c3g-human-serum-metabolite-signatures","display_name":"Reconstructed 1-, 6- and 24-hour human C3G metabolite signatures","entity_type_key":"chemical_species"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Full text retrieved; relevant methods/results/figure text reviewed. No independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"In vitro mixtures reconstructed with authentic standards from a human tracer cohort.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Preparation provenance does not attribute every component effect to the parent or transfer unrelated metabolite findings.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The tested signatures reconstruct serum metabolite profiles measured after labeled C3G exposure; they are separate mixtures, not intact C3G.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Signatures of anthocyanin metabolites identified in humans inhibit biomarkers of vascular inflammation in human endothelial cells. | 2017 | DOI 10.1002/mnfr.201700053 | PMID 28457017 | https://pubmed.ncbi.nlm.nih.gov/28457017/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC5600085/ | https://doi.org/10.1002/mnfr.201700053","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 58-58; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"cc41c009-3b36-57a1-b188-98ab43227013","evidence_kind":"source_excerpt","locator":"Lines 58-58","start_line":58,"end_line":58,"excerpt":"**Low-concentration mixtures can change VCAM-1 without a universal IL-6 effect.** Warner's 2017 study reconstructed the tracer cohort's 1-, 6- and 24-hour serum signatures. Mixtures reduced TNF-stimulated VCAM-1; some effects remained at total concentrations of 0.19–0.44 µM. IL-6 secretion decreased in HUVECs at selected higher mixture concentrations, but not detectably in HCAECs. TNF stimulation also differed between the cell types. Figure 3 preserves this important cell-specific null. Phosphorylated NF-κB p65 did not significantly change in the tested HCAEC experiment. These are mixture findings, not proof that PCA alone suppresses NF-κB at dietary exposure. [Warner et al., 2017, Figures 2–5](https://pmc.ncbi.nlm.nih.gov/articles/PMC5600085/).","model_system":"In vitro mixtures reconstructed with authentic standards from a human tracer cohort.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b08e7f7d-4d34-56d7-a185-2124f8d72b3c","stable_key":"import-35ec55a7-323c-5c28-979e-3bdafe9d5769","title":"Anthocyanins: detailed mechanisms of action (reviewed 4 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"7cff1a47fbd9c625412b84162b1c823004b4162b7c009f9a11d5807fb8e04ef9","revision_id":"1bd4fa42-bfeb-5148-8250-fc363d8c1de0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}