{"id":"fc19c8ed-38e5-5116-9f02-e39066a8221f","stable_key":"53d9b98d-e3a0-5416-bc88-adbe42a8cb1b:curcumin-keap-mutant","predicate":"attenuates","statement":"The C151S construct reduced curcumin-induced Nrf2 transactivation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"07e8731c-1986-58e1-82e7-51398b73c0b0","mechanism_event_label":"Changing one sensor residue weakened the response.","subject":{"id":"f4996e18-36b5-5d61-af5f-1b6bf284d770","slug":"curcumin-keap1-c151s-construct","display_name":"KEAP1 C151S construct in the 2020 curcumin study","entity_type_key":"protein_state"},"object":{"id":"b1df6f55-a208-547c-a271-77e4edbb92eb","slug":"mouse-nrf2-transactivation","display_name":"Nrf2-dependent transcription in murine epidermal-cell assay","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"07e8731c-1986-58e1-82e7-51398b73c0b0","stable_key":"53d9b98d-e3a0-5416-bc88-adbe42a8cb1b:curcumin-keap-mutant-event","event_type":"biochemical_relationship","label":"Changing one sensor residue weakened the response.","description":"The C151S construct reduced curcumin-induced Nrf2 transactivation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0e625439-d093-5d1b-8713-dcfdafcf6811","slug":"curcumin","display_name":"Curcumin","entity_type_key":"small_molecule"},"role":"stimulus","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f4996e18-36b5-5d61-af5f-1b6bf284d770","slug":"curcumin-keap1-c151s-construct","display_name":"KEAP1 C151S construct in the 2020 curcumin study","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b1df6f55-a208-547c-a271-77e4edbb92eb","slug":"mouse-nrf2-transactivation","display_name":"Nrf2-dependent transcription in murine epidermal-cell assay","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/curcumin-research/31972171.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0a05e877dfe677824dea390c30634719eba641ed122c2480147ce2e73bf05936\", \"start_char\": 0, \"end_char\": 1591, \"text_sha256\": \"0a05e877dfe677824dea390c30634719eba641ed122c2480147ce2e73bf05936\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse epidermal-cell and skin experiments, siRNA and receptor-construct assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Curcumin versus tetrahydrocurcumin; Nrf2 siRNA and Keap1 C151S construct","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell exposures and construct-origin details are not available in the indexed abstract. Results do not establish human systemic target engagement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Curcumin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"curcumin","display_name":"Curcumin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mus musculus; transfected construct species not resolved in indexed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Changing one sensor residue weakened the response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[curcumin-p31972171] Curcumin induces stabilization of Nrf2 protein through Keap1 cysteine modification. (2020). https://pubmed.ncbi.nlm.nih.gov/31972171/ DOI: 10.1016/j.bcp.2020.113820","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"JB6 epidermal cells and mouse skin","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"2d4c56a4-426f-52ec-a74d-0312c375ca2d","evidence_kind":"source_excerpt","locator":"Lines 424-435","start_line":424,"end_line":435,"excerpt":"### curcumin-keap-mutant\nThe C151S construct reduced curcumin-induced Nrf2 transactivation.\nCondition category: machinery_impairment\nnutrient_topic: Curcumin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Changing one sensor residue weakened the response.\norganism: Mus musculus; transfected construct species not resolved in indexed abstract\ntissue_or_cell_type: JB6 epidermal cells and mouse skin\nexperimental_model: Mouse epidermal-cell and skin experiments, siRNA and receptor-construct assays\nlimitations: Cell exposures and construct-origin details are not available in the indexed abstract. Results do not establish human systemic target engagement.\nexposure: Curcumin versus tetrahydrocurcumin; Nrf2 siRNA and Keap1 C151S construct\nevidence_span: {\"source_cache\": \"artifacts/curcumin-research/31972171.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0a05e877dfe677824dea390c30634719eba641ed122c2480147ce2e73bf05936\", \"start_char\": 0, \"end_char\": 1591, \"text_sha256\": \"0a05e877dfe677824dea390c30634719eba641ed122c2480147ce2e73bf05936\"}\n[curcumin-p31972171] Curcumin induces stabilization of Nrf2 protein through Keap1 cysteine modification. (2020). https://pubmed.ncbi.nlm.nih.gov/31972171/ DOI: 10.1016/j.bcp.2020.113820","model_system":"Mouse epidermal-cell and skin experiments, siRNA and receptor-construct assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [curcumin-p31972171] Curcumin induces stabilization of Nrf2 protein through Keap1 cysteine modification. (2020). https://pubmed.ncbi.nlm.nih.gov/31972171/ DOI: 10.1016/j.bcp.2020.113820","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"11d29ea9-3af1-5404-a68d-6f129e23edee","stable_key":"import-53d9b98d-e3a0-5416-bc88-adbe42a8cb1b","title":"Curcumin: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"6eeda0e6e358e0b19f73f554f8c027cb08015aa49d92a77bde36e3fb3ea1a41c","revision_id":"32e73d69-5445-5ace-b25d-5a4d3787aea5","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}