{"id":"fc14801d-7000-5ec8-9f17-88e06edd874e","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-gut-trpm5","predicate":"contributes_to","statement":"The human HuTu-80 experiments also supported TRPM5 involvement in rebaudioside-A-evoked GLP-1 release.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0a50183a-6ff2-5335-97b1-445e1bec54bc","mechanism_event_label":"Taste-associated ion-channel machinery is shared with intestinal signaling.","subject":{"id":"b408a655-b79c-56c9-bbdd-78cd3ad7ac65","slug":"trpm5","display_name":"Human calcium-activated monovalent cation channel / TRPM5","entity_type_key":"protein"},"object":{"id":"46731938-4c86-5195-b463-c14c70168dfc","slug":"human-hutu80-glp1-release","display_name":"Human HuTu-80 GLP-1 release","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0a50183a-6ff2-5335-97b1-445e1bec54bc","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-gut-trpm5-event","event_type":"observed_relationship","label":"Taste-associated ion-channel machinery is shared with intestinal signaling.","description":"The human HuTu-80 experiments also supported TRPM5 involvement in rebaudioside-A-evoked GLP-1 release.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b408a655-b79c-56c9-bbdd-78cd3ad7ac65","slug":"trpm5","display_name":"Human calcium-activated monovalent cation channel / TRPM5","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"46731938-4c86-5195-b463-c14c70168dfc","slug":"human-hutu80-glp1-release","display_name":"Human HuTu-80 GLP-1 release","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c78086c3-7657-589b-b353-1691df2d9f8f","slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"16a5658c-2844-5742-9d3b-8882e5414eb6","slug":"rebaudioside-a","display_name":"Rebaudioside A","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"4853afc6-9eaa-547f-91d7-c4dd90082351","slug":"glp-1","display_name":"Glucagon-like peptide 1 / GLP-1","entity_type_key":"peptide"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human enteroendocrine cell-line signaling experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The species-specific human protein node is distinct from mouse Trpm5 and the incompletely resolved expression construct.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Stevia collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Taste-associated ion-channel machinery is shared with intestinal signaling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Rebaudioside A from Stevia rebaudiana stimulates GLP-1 release by enteroendocrine cells via bitter taste signalling pathways. · 2023 · https://pubmed.ncbi.nlm.nih.gov/37431625/ · DOI 10.1039/d3fo00818e","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d60a1c42-4d3c-54b4-ab19-57dbb8d34e8f","evidence_kind":"source_excerpt","locator":"Lines 274-280","start_line":274,"end_line":280,"excerpt":"## stevia-gut-trpm5\nTaste-associated ion-channel machinery is shared with intestinal signaling.\nThe human HuTu-80 experiments also supported TRPM5 involvement in rebaudioside-A-evoked GLP-1 release.\nModel: Human enteroendocrine cell-line signaling experiments.\nLimitations: The species-specific human protein node is distinct from mouse Trpm5 and the incompletely resolved expression construct.\nEvidence access: Primary abstract\nRebaudioside A from Stevia rebaudiana stimulates GLP-1 release by enteroendocrine cells via bitter taste signalling pathways. · 2023 · https://pubmed.ncbi.nlm.nih.gov/37431625/ · DOI 10.1039/d3fo00818e","model_system":"Human enteroendocrine cell-line signaling experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; 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