{"id":"fbe34d4f-a4a6-58f6-82d2-abb2ab4f0e07","stable_key":"bf161ddf-8b82-5d65-b86d-0b41245aac61:phlorizin-vanadate-comparator","predicate":"normalizes_indirectly","statement":"Phlorizin and vanadate both prevented diabetes-associated hepatic insulin-receptor overexpression in vivo, but only vanadate directly lowered transcripts in hepatoma cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"04adec43-2db8-5110-8f8e-cbcae6101b8c","mechanism_event_label":"Phlorizin and vanadate both prevented diabetes-associated hepatic insulin-receptor overexpression in vivo, but only vanadate directly lowered transcripts in hepatoma cells.","subject":{"id":"3516a0ba-1f72-5e1b-bccb-7fca992f96f7","slug":"phlorizin","display_name":"Phlorizin","entity_type_key":"small_molecule"},"object":{"id":"f164e072-d71a-59b5-bab6-783bfe2380dc","slug":"diabetic-rat-liver-insulin-receptor-expression","display_name":"Liver insulin-receptor expression in streptozotocin-diabetic rats","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"04adec43-2db8-5110-8f8e-cbcae6101b8c","stable_key":"bf161ddf-8b82-5d65-b86d-0b41245aac61:phlorizin-vanadate-comparator-event","event_type":"observed_relationship","label":"Phlorizin and vanadate both prevented diabetes-associated hepatic insulin-receptor overexpression in vivo, but only vanadate directly lowered transcripts in hepatoma cells.","description":"Phlorizin and vanadate both prevented diabetes-associated hepatic insulin-receptor overexpression in vivo, but only vanadate directly lowered transcripts in hepatoma cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3516a0ba-1f72-5e1b-bccb-7fca992f96f7","slug":"phlorizin","display_name":"Phlorizin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f164e072-d71a-59b5-bab6-783bfe2380dc","slug":"diabetic-rat-liver-insulin-receptor-expression","display_name":"Liver insulin-receptor expression in streptozotocin-diabetic rats","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c6cbaee8-ccf3-5f2b-a672-433a1c915360","slug":"vanadate","display_name":"Vanadate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"5cd18880-8dc7-5897-9362-7422684f5f79","slug":"insr","display_name":"Insulin receptor / INSR","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Daily phlorizin or vanadate from days 5-23; direct cell exposures","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"18 days in rats; 4 hours in cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Streptozotocin-diabetic rats and Fao hepatoma cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The contrast supports an indirect glycemia route for phlorizin and a distinct direct action for vanadate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Phlorizin chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"phlorizin","display_name":"Phlorizin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Streptozotocin-diabetic rats and Fao hepatoma cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Phlorizin and vanadate both prevented diabetes-associated hepatic insulin-receptor overexpression in vivo, but only vanadate directly lowered transcripts in hepatoma cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Treatment of streptozotocin-induced diabetic rats with vanadate and phlorizin prevents the over-expression of the liver insulin receptor gene. (1999). https://pubmed.ncbi.nlm.nih.gov/10037256/ DOI: 10.1530/eje.0.1400079","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vivo and in vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Insulin-receptor number and mRNA","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4cb5e99c-b88b-55c2-a951-5472364b0484","evidence_kind":"source_excerpt","locator":"Lines 132-141","start_line":132,"end_line":141,"excerpt":"## phlorizin-vanadate-comparator\nPhlorizin and vanadate both prevented diabetes-associated hepatic insulin-receptor overexpression in vivo, but only vanadate directly lowered transcripts in hepatoma cells.\nModel/species: Streptozotocin-diabetic rats and Fao hepatoma cells\nTissue/system: Insulin-receptor number and mRNA\nExposure: Daily phlorizin or vanadate from days 5-23; direct cell exposures\nRoute: In vivo and in vitro\nDuration: 18 days in rats; 4 hours in cells\nLimits: The contrast supports an indirect glycemia route for phlorizin and a distinct direct action for vanadate.\nPrimary reference: Treatment of streptozotocin-induced diabetic rats with vanadate and phlorizin prevents the over-expression of the liver insulin receptor gene. (1999). https://pubmed.ncbi.nlm.nih.gov/10037256/ DOI: 10.1530/eje.0.1400079\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Streptozotocin-diabetic rats and Fao hepatoma cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7d4bfcb9-1665-5686-8b8a-1ad17ad6a4d2","stable_key":"import-bf161ddf-8b82-5d65-b86d-0b41245aac61","title":"Phlorizin: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"13aa8f888801708147608784e74ad34d625b10fd76ea5afd542a3f9328bd40e2","revision_id":"6997ff77-8628-59b7-878c-95fed9d6a622","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}